JOVE JOURNAL

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A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii

Jennifer L. Hoover¹, Thomas F. Lewandowski¹, Cindy L. Mininger¹, Christine M. Singley¹, Scott Sucoloski¹, Stephen Rittenhouse¹

¹Antibacterial Discovery Performance Unit,GlaxoSmithKline

Journal (Immunology and Infection)

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

Tik Nga Tong¹, Donald R. Branch^1,2

¹Department of Laboratory Medicine and Pathobiology,University of Toronto, ²Centre for Innovation,Canadian Blood Services

Journal (Immunology and Infection)

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Pamela Holzlöhner¹, Katja Hanack¹

¹Department of Biochemistry and Biology,University of Potsdam

Journal (Immunology and Infection)

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Angelika Schmidt¹, Szabolcs Éliás*¹, Rubin N. Joshi*¹, Jesper Tegnér¹

¹Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna,Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

Journal (Immunology and Infection)

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Jennifer N. Gibson¹, Pavani Beesetty¹, Courtney Sulentic², J. Ashot Kozak¹

¹Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine,Wright State University, ²Department of Pharmacology and Toxicology, Boonshoft School of Medicine,Wright State University

Journal (Immunology and Infection)

A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries

Malena E. Skogman¹, Pia M. Vuorela¹, Adyary Fallarero¹

¹Pharmaceutical Design and Discovery (PharmDD), Faculty of Pharmacy,University of Helsinki

Journal (Immunology and Infection)

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Wilfried Posch¹, Cornelia Lass-Flörl¹, Doris Wilflingseder¹

¹Division of Hygiene and Medical Microbiology,Medical University of Innsbruck

Journal (Immunology and Infection)

Analysis of Simian Immunodeficiency Virus-specific CD8⁺ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Lucas Gonzalez-Nieto¹, Aline Domingues¹, Michael Ricciardi¹, Martin J. Gutman¹, Helen S. Maxwell¹, Nuria Pedreño-Lopez¹, Varian Bailey¹, Diogo M. Magnani¹, Mauricio A. Martins¹

¹Patoloji Bölümü,University of Miami

Journal (Immunology and Infection)

Optimization of a Quantitative Micro-neutralization Assay

Yipu Lin¹, Yan Gu¹, John W. McCauley¹

¹Mill Hill Laboratory,The Francis Crick Institute

Journal (Immunology and Infection)

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model

Trevor Beaudoin¹, Sarah Kennedy², Yvonne Yau³, Valerie Waters⁴

¹Physiology and Experimental Medicine,Hospital for Sick Children, ²Department of Clinical Microbiology,Royal College of Surgeons in Ireland, ³Department of Laboratory Medicine and Pathobiology,University of Toronto, ⁴Pediatri Anabilim Dalı,University of Toronto

Journal (Immunology and Infection)

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Ben Gold¹, Julia Roberts*¹, Yan Ling*¹, Landys Lopez Quezada¹, Jou Glasheen¹, Elaine Ballinger¹, Selin Somersan-Karakaya², Thulasi Warrier¹, Carl Nathan¹

¹Departments of Microbiology & Immunology,Weill Cornell Medical College, ²Tıp,Weill Cornell Medical College

Journal (Immunology and Infection)

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Shiang-Jong Tzeng¹

¹Graduate Institute of Pharmacology, College of Medicine,National Taiwan University

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