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Journal (Developmental Biology)

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Sergey Kurdyukov¹, Youhong Song¹, Terence W-Y Tiew¹, Xin-Ding Wang¹, Kim E. Nolan¹, Ray J. Rose¹

¹School of Environmental and Life Sciences,University of Newcastle, Callaghan, New South Wales, Australia

Journal (Developmental Biology)

Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment

Janice L. Walker¹, Brigid M. Bleaken¹, Iris M. Wolff¹, A. Sue Menko¹

¹Pathology, Anatomy and Cell Biology,Thomas Jefferson University

Journal (Developmental Biology)

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Viviana Meraviglia*¹, Alessandra Zanon*¹, Alexandros A. Lavdas¹, Christine Schwienbacher¹, Rosamaria Silipigni², Marina Di Segni², Huei-Sheng Vincent Chen³, Peter P. Pramstaller¹, Andrew A. Hicks¹, Alessandra Rossini¹

¹Center for Biomedicine,European Academy Bozen/Bolzano (EURAC), ²Laboratory of Medical Genetics,Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, ³Del E. Webb Center for Neuroscience, Aging & Stem Cell Research,Sanford-Burnham Medical Research Institute

Journal (Developmental Biology)

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Shenglan Li*^1,2, Haipeng Xue*^1,2, Bo Long^1,2,5, Li Sun^1,2,6, Tai Truong^1,2,4,7, Ying Liu^1,2,3

¹Department of Neurosurgery,The University of Texas Health Science Center at Houston, ²Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,The University of Texas Health Science Center at Houston, ³The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine,The University of Texas Health Science Center at Houston, ⁴Summer Research Program, Office of Educational Programs,The University of Texas Health Science Center at Houston, ⁵Department of Anesthesiology,Shengjing Hospital, China Medical University, ⁶Department of Oncology, Renji Hospital,Shanghai Jiaotong University School of Medicine, ⁷Biology Department,University of West Georgia

Journal (Developmental Biology)

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick^GFPNeural Tube Grafting and Carbocyanine Dye DiI Injection

Jean-Marie Delalande^1,2, Nikhil Thapar¹, Alan J. Burns^1,3

¹Birth Defects Research Centre,UCL Institute of Child Health, ²Blizard Institute, Centre for Digestive Diseases,Queen Mary University of London, Barts and The London School of Medicine and Dentistry, ³Department of Clinical Genetics,Erasmus University Medical Center, Rotterdam

Journal (Developmental Biology)

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Tae-Young Choi¹, Mehwish Khaliq¹, Sungjin Ko¹, Juhoon So¹, Donghun Shin¹

¹Department of Developmental Biology, McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Wikrom Wongpaiboonwattana¹, Marios P. Stavridis¹

¹Division of Cancer Research, College of Medicine, Dentistry and Nursing,University of Dundee

Journal (Developmental Biology)

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Michael K. Conway¹, Michael J. Gerger¹, Erin E. Balay¹, Rachel O'Connell¹, Seth Hanson², Neil J. Daily¹, Tetsuro Wakatsuki¹

¹InvivoSciences, Inc., ²Gilson, Inc.

Journal (Developmental Biology)

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Andrea Mothe¹, Charles H. Tator^1,2

¹Division of Genetics and Development,Toronto Western Research Institute and Krembil Neuroscience Center, ²Department of Surgery, Division of Neurosurgery,Toronto Western Hospital and University of Toronto

Journal (Developmental Biology)

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Daniel Prieto¹, Gonzalo Aparicio^1,2, Matías Machado¹, Flavio R. Zolessi^1,2

¹Institut Pasteur de Montevideo, ²Sección Biología Celular, Facultad de Ciencias,Universidad de la República

Journal (Developmental Biology)

Immunostaining to Visualize Murine Enteric Nervous System Development

Amanda J. Barlow-Anacker¹, Christopher S. Erickson¹, Miles L. Epstein², Ankush Gosain^1,2,3

¹Cerrahi Bölümü,University of Wisconsin School of Medicine and Public Health, ²Department of Neurosciences,University of Wisconsin School of Medicine and Public Health, ³Pediatri Anabilim Dalı,University of Wisconsin School of Medicine and Public Health

Journal (Developmental Biology)

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies

Daniel Vogt¹, Pei-Rung Wu¹, Shawn F. Sorrells², Christine Arnold², Arturo Alvarez-Buylla², John L. R. Rubenstein¹

¹Psikiyatri Bölümü,University of California San Francisco, ²Department of Neurological Surgery,University of California San Francisco

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