JOVE JOURNAL

Biyoloji

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CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Kyle E. Simpson¹, Shoaib Faizi¹, Ravichandra Venkateshappa¹, Mandy Yip¹, Raj Johal¹, Damon Poburko¹, Yen May Cheng¹, Diana Hunter¹, Eric Lin¹, Glen F. Tibbits¹, Thomas W. Claydon¹

¹Department of Biomedical Physiology and Kinesiology,Simon Fraser University

Journal (Biology)

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

Rihab Bouchareb¹, Liping Yu¹, Emelie Lassen¹, Ilse S. Daehn¹

¹Department of Medicine, Division of Nephrology,The Icahn School of Medicine at Mount Sinai

Journal (Biology)

Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis

Arvind Sharma¹, Michael Pham¹, Robert A. Harrell II², Andrew B. Nuss³, Monika Gulia-Nuss¹

¹Biyokimya ve Moleküler Biyoloji Anabilim Dalı,University of Nevada, Reno, ²Insect Transformation Facility,University of Maryland Institute for Bioscience and Biotechnology Research, ³Department of Agriculture, Veterinary, and Rangeland Sciences,University of Nevada, Reno

Journal (Biology)

Hand Dissection of Caenorhabditis elegans Intestines

Jessica L. Hill¹, Andrew Moore¹, Robert T. P. Williams¹, Erin Osborne Nishimura¹

¹Department of Biochemistry & Molecular Biology,Colorado State University

Journal (Biology)

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

Vaibhav Bhardwaj*¹, Kumari Sweta*¹, Deepak Gyala¹, Nimisha Sharma¹

¹University School of Biotechnology, G.G.S. Indraprastha University

Journal (Biology)

Embryo Rescue Protocol for Interspecific Hybridization in Squash

Yuqing Fu¹, Swati Shrestha¹, Pamela Moon¹, Geoffrey Meru¹

¹UF/IFAS Tropical Research and Education Center

Journal (Biology)

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

Chun Wang*¹, Jie Lv*¹, Wei Zhuang^1,2, Ling Xie¹, Guangyu Liu¹, Kaidireya Saimaier¹, Sanxing Han¹, Changjie Shi¹, Qiuhong Hua¹, Ru Zhang¹, Guangfeng Shi³, Changsheng Du¹

¹Putuo People's Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology,Tongji University, ²National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics,Chinese Academy of Sciences, ³Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital,Fudan University

Journal (Biology)

Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images

Deepak Kunhi Valappil*¹, Abinaya Raghavan*¹, Sangeeta Nath¹

¹Manipal Institute of Regenerative Medicine,Manipal Academy of Higher Education, India

Journal (Biology)

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Doudou Huang*¹, Haixia Ma*¹, Lu Zhao^1,2, Xiaoyu Wang^1,2, Yi Huang^1,2, Fei Wang¹, Zhiming Yuan^1,2, Han Xia^1,2

¹Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology,Chinese Academy of Sciences, ²University of Chinese Academy of Sciences

Journal (Biology)

Collection and Long-Term Maintenance of Leaf-Cutting Ants (Atta) in Laboratory Conditions

Bianca Raissa Nogueira¹, Amanda Aparecida de Oliveira¹, Janiele Pereira da Silva², Odair Correa Bueno¹

¹Institute of Biosciences, Department of General and Applied Biology, Laboratory of Leaf-cutting Ants,São Paulo State University, ²Institute of Psychology, Laboratory Walter Hugo Cunha,São Paulo University

Journal (Biology)

Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays

Vlad Stoian¹, Roxana Vidican¹, Larisa Corcoz¹, Victoria Pop-Moldovan¹

¹Department of Microbiology, Faculty of Agriculture,University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Journal (Biology)

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Mehrzad Sasanpour¹, Daisy H. Achiriloaie^1,2, Gloria Lee¹, Gregor Leech¹, Maya Hendija¹, K. Alice Lindsay³, Jennifer L. Ross³, Ryan J. McGorty¹, Rae M. Robertson-Anderson¹

¹Department of Physics and Biophysics,University of San Diego, ²W. M. Keck Science Department,Scripps College, Pitzer College, and Claremont McKenna College, ³Fizik Bölümü,Syracuse University

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