JOVE JOURNAL

Biyoloji

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Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program

Bianca J. Moreno¹, Robert L. Aldridge¹, Seth C. Britch¹, Barbara E. Bayer¹, Jedidiah Kline¹, Daniel A. Hahn², Chao Chen², Kenneth J. Linthicum¹

¹US Department of Agriculture,Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology, ²Department of Entomology and Nematology,Florida Üniversitesi

Journal (Biology)

Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition

Samuel A. Sprowls*¹, Vincenzo J. Pizzuti*^1,3, William Pentz¹, Divine C. Nwafor^3,4, R. Alfredo C. Siochi^2,3, Paul R. Lockman¹

¹Department of Basic Pharmaceutical Sciences, School of Pharmacy,West Virginia University, ²Radiation Oncology,West Virginia University, ³School of Medicine,West Virginia University, ⁴Nörobilim Bölümü,West Virginia University

Journal (Biology)

A Step-by-Step Guide to Mosquito Electroantennography

Chloé Lahondère¹

¹Department of Biochemistry; The Fralin Life Science Institute; The Global Change Center; Department of Entomology and the Center for Emerging Zoonotic and Arthropod-Borne Pathogens,Virginia Polytechnic Institute and State University

Journal (Biology)

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

Ying Wu*¹, Darwin Diaz*¹, Jian Yin¹, David Bloodgood¹, William Sexton¹, Cheng-I Wei², Shunyuan Xiao^1,3

¹Institute for Bioscience and Biotechnology Research,University of Maryland, ²Department of Nutrition and Food Science,University of Maryland College Park, ³Department of Plant Sciences and Landscape Architecture,University of Maryland College Park

Journal (Biology)

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Daniel W. Weisgerber¹, Makiko N. Hatori¹, Adam R. Abate^1,2

¹Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences,University of California, ²Chan Zuckerberg Biohub

Journal (Biology)

Field-Based Thermal Physiology Assay: Cold Shock Recovery under Ambient Conditions

Emily S. Khazan¹

¹School of Natural Resources and Environment,Florida Üniversitesi

Journal (Biology)

In vivo Measurement of Knee Extensor Muscle Function in Mice

Camille R. Brightwell^1,2, Ted G. Graber³, Benjamin D. Brightwell^4,5, Matthew Borkowski⁶, Brian Noehren^5,7, Christopher S. Fry^1,2

¹Department of Athletic Training and Clinical Nutrition,Kentucky Üniversitesi, ²Center for Muscle Biology,Kentucky Üniversitesi, ³Department of Physical Therapy,East Carolina University, ⁴Kinesiology and Health Promotion Graduate Program,Kentucky Üniversitesi, ⁵Biomotion Lab, College of Health Sciences,Kentucky Üniversitesi, ⁶Aurora Scientific, ⁷Department of Physical Therapy, College of Health Sciences,Kentucky Üniversitesi

Journal (Biology)

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

Lauren S. Godwin¹, Emily Roberts², Joanna M. Bridger¹, Helen A. Foster²

¹Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences,Brunel University London, ²Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences,University of Hertfordshire

Journal (Biology)

Assessing Agrochemical Risk to Mated Honey Bee Queens

Julia D. Fine¹, Kendall M. Torres², Jamilyn Martin², Gene E. Robinson^2,3,4

¹Invasive Species and Pollinator Health Research Unit,USDA-ARS, ²Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, ³Neuroscience Program,University of Illinois at Urbana-Champaign, ⁴Entomoloji Anabilim Dalı,University of Illinois at Urbana-Champaign

Journal (Biology)

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Bliss M. Beernink*¹, Katerina L. Holan*¹, Ryan R. Lappe¹, Steven A. Whitham¹

¹Department of Plant Pathology and Microbiology,Iowa State Üniversitesi

Journal (Biology)

TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Jin Ma^1,2, Gerard van der Zon^1,2, Gonzalo Sanchez-Duffhues¹, Peter ten Dijke^1,2

¹Dept. Cell Chemical Biology,Leiden Üniversitesi Tıp Merkezi, ²Oncode Institute,Leiden Üniversitesi Tıp Merkezi

Journal (Biology)

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Thais Bascuas*^1,2, Martina Kropp*^1,2, Nina Harmening^1,2, Brittany M. Wong¹, Sandra Johnen³, Zsuzsanna Izsvák⁴, Gabriele Thumann^1,2

¹Experimental Ophthalmology,University of Geneva, ²Department of Ophthalmology,University Hospitals of Geneva, ³Department of Ophthalmology,University Hospital RWTH Aachen, ⁴Max Delbrück Center for Molecular Medicine

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