JOVE JOURNAL

Biyoloji

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High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Natalie J. Saez¹, Hervé Nozach², Marilyne Blemont¹, Renaud Vincentelli¹

¹Architecture et Fonction des Macromolécules Biologiques (AFMB),Aix-Marseille Université, ²iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO),Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Saclay, France

Journal (Biology)

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Jelle Caers¹, Katleen Peymen¹, Nick Suetens¹, Liesbet Temmerman¹, Tom Janssen¹, Liliane Schoofs¹, Isabel Beets¹

¹Biyoloji Bölümü,KU Leuven

Journal (Biology)

Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam

Stefano Rubino^1,4, Petter Melin³, Paul Spellward², Klaus Leifer¹

¹Department of Engineering Sciences,Uppsala University, ²Gatan Inc., ³Department of Microbiology,Swedish University of Agricultural Sciences, ⁴Physics Department,University of Oslo

Journal (Biology)

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands

Rushin Shojaii¹, Stephanie Bacopulos^2,3, Wenyi Yang^2,3, Tigran Karavardanyan⁴, Demetri Spyropoulos⁵, Afshin Raouf⁶, Anne Martel^1,4, Arun Seth^2,3

¹Department of Medical Biophysics,University of Toronto, ²Platform Biological Sciences,Sunnybrook Research Institute, ³Department of Laboratory Medicine and Pathobiology,University of Toronto, ⁴Physical Sciences,Sunnybrook Research Institute, ⁵Department of Pathology and Laboratory Medicine,Medical University of South Carolina, ⁶Manitoba Institute of Cell Biology,University of Manitoba

Journal (Biology)

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Kevin G. Chen¹, Rebecca S. Hamilton¹, Pamela G. Robey², Barbara S. Mallon¹

¹NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health, ²Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research,National Institutes of Health

Journal (Biology)

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions

Alexis Peaucelle^1,2

¹Laboratoire Matière et Systèmes Complexes, UFR de Physique,Université Paris Diderot, ²Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech,INRA Centre de Versailles-Grignon

Journal (Biology)

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

Alexis B. Webb¹, Daniele Soroldoni¹, Annelie Oswald¹, Johannes Schindelin¹, Andrew C. Oates¹

¹Max Planck Institute of Molecular Cell Biology and Genetics

Journal (Biology)

SIVQ-LCM Protocol for the ArcturusXT Instrument

Jason D. Hipp*¹, Jerome Cheng*², Jeffrey C. Hanson*¹, Avi Z. Rosenberg¹, Michael R. Emmert-Buck¹, Michael A. Tangrea¹, Ulysses J. Balis²

¹Laboratory of Pathology, National Cancer Institute,National Institutes of Health, ²Patoloji Bölümü,University of Michigan

Journal (Biology)

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Houtan Moshiri*^1,2, Vaibhav Mehta*^1,2, Reza Salavati^1,2,3

¹Department of Biochemistry,McGill University, ²Institute of Parasitology,McGill University, ³McGill Centre for Bioinformatics,McGill University

Journal (Biology)

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Lara Rajeev¹, Eric G. Luning¹, Aindrila Mukhopadhyay¹

¹Physical Biosciences Division,Lawrence Berkeley National Laboratory

Journal (Biology)

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Landon J. Inge¹, Aaron J. Fowler¹, Ross M. Bremner¹

¹Center for Thoracic Disease and Transplantation, Heart and Lung Institute,St. Joseph's Hospital and Medical Center

Journal (Biology)

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

Chiara Greggio*¹, Filippo De Franceschi*¹, Manuel Figueiredo-Larsen*², Anne Grapin-Botton^1,2

¹Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences,Swiss Institute for Experimental Cancer Research, ²DanStem,University of Copenhagen

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