JOVE JOURNAL

Biyoloji

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Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Thimma R. Reddy¹, Emma J. Kelsall¹, Léna M.S. Fevat², Sarah E. Munson*³, Shaun M. Cowley*¹

¹Department of Biochemistry,University of Leicester, ²Center for Fisheries, Environment and Aquaculture Sciences, ³ES Cell Facility, Centre for Core Biotechnology Services,University of Leicester

Journal (Biology)

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Louise E. Bird¹, Heather Rada¹, Anil Verma¹, Raphael Gasper^2,3, James Birch⁴, Matthew Jennions⁴, Jan Lӧwe³, Isabel Moraes⁴, Raymond J. Owens¹

¹Oxford Protein Production Facility,Research Complex at Harwell, ²Protein Crystallography Group,Ruhr University, ³MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, ⁴Membrane Protein Laboratory,Diamond Light Source

Journal (Biology)

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Amanda M. Larracuente¹, Patrick M. Ferree²

¹Biyoloji Bölümü,University of Rochester, ²W. M. Keck Science Department,Claremont McKenna, Pitzer, and Scripps Colleges

Journal (Biology)

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

Nathachit Limjunyawong¹, Jonathan Fallica¹, Amritha Ramakrishnan², Kausik Datta³, Matthew Gabrielson¹, Maureen Horton¹, Wayne Mitzner¹

¹Department of Environmental Health Sciences,Johns Hopkins University Bloomberg School of Public Health, ²Department of International Health,Johns Hopkins University Bloomberg School of Public Health, ³Tıp Fakültesi,Johns Hopkins University School of Medicine

Journal (Biology)

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Daniel E. Bauer*^1,2,3, Matthew C. Canver*¹, Stuart H. Orkin^1,2,3,4

¹Harvard Tıp Fakültesi, ²Division of Hematology/Oncology,Boston Children's Hospital, ³Department of Pediatric Oncology,Dana-Farber Cancer Institute, ⁴Howard Hughes Medical Institute

Journal (Biology)

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

Lin Zhang¹, Katleen Vranckx², Koen Janssens², Todd R. Sandrin¹

¹School of Mathematical and Natural Sciences,Arizona State University, ²Applied Maths NV

Journal (Biology)

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Dexin Sui*¹, Mengyu Liu*¹, Min-Hao Kuo¹

¹Biyokimya ve Moleküler Biyoloji Anabilim Dalı,Michigan State University

Journal (Biology)

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

Jackson T. Sparks¹, Joseph C. Dickens¹

¹Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Plant Sciences Institute, Invasive Insect Biocontrol and Behavior Laboratory,United States Department of Agriculture

Journal (Biology)

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

Mark A. Baker¹, Louise Hetherington¹, Anita Weinberg¹, Tony Velkov²

¹School of Environmental and Life Science,University of Newcastle, ²Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences,Monash University

Journal (Biology)

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Yan Wei Lim¹, Matthew Haynes², Mike Furlan¹, Charles E. Robertson³, J. Kirk Harris⁴, Forest Rohwer¹

¹Biyoloji Bölümü,San Diego State University, ²DOE Joint Genome Institute, ³Department of Molecular, Cellular and Developmental Biology,University of Colorado, ⁴Department of Pediatrics, School of Medicine,University of Colorado

Journal (Biology)

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Shubhra Majumder¹, Harold A. Fisk¹

¹Department of Molecular Genetics,The Ohio State University

Journal (Biology)

Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

Nasmah K. Bastaki¹, Christopher A. Cullis¹

¹Biyoloji Bölümü,Case Western Reserve University

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