JOVE JOURNAL

Biyokimya

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Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Joanna Kud¹, Wenjie Wang², Yulin Yuan², Allan Caplan², Joseph C. Kuhl², Louise-Marie Dandurand¹, Fangming Xiao²

¹Department of Entomology, Plant Pathology and Nematology,University of Idaho, ²Department of Plant Sciences,University of Idaho

Journal (Biochemistry)

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Keni Vidilaseris*¹, Niklas G. Johansson*², Ainoleena Turku², Alexandros Kiriazis², Gustav Boije af Gennäs², Jari Yli-Kauhaluoma², Henri Xhaard², Adrian Goldman^1,3

¹Research Program in Molecular and Integrative Biosciences,University of Helsinki, ²Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy,University of Helsinki, ³School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology,University of Leeds

Journal (Biochemistry)

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling

Steven E. Guard¹, Christopher C. Ebmeier¹, William M. Old^1,2

¹Department of Molecular, Cellular and Developmental Biology,University of Colorado, ²Linda Crnic Institute for Down Syndrome,University of Colorado School of Medicine

Journal (Biochemistry)

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

David André Coucheron¹, Øystein Ivar Helle¹, Cristina Ionica Øie², Jean-Claude Tinguely¹, Balpreet Singh Ahluwalia¹

¹Department of Physics and Technology,UiT The Arctic University of Norway, ²Vascular Biology Research Group, Department of Medical Biology,UiT The Arctic University of Norway

Journal (Biochemistry)

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Ryan K. Messer¹, Miguel A. Lopez Jr.¹, Gayan Senavirathne¹, Kristine E. Yoder¹

¹Cancer Biology and Genetics, College of Medicine,The Ohio State University

Journal (Biochemistry)

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Mirna Swayden¹, Aurélie Dobric¹, Yolande Berthois¹, Hugo Villalba¹, Stéphane Audebert¹, Luc Camoin¹, Juan Iovanna¹, Philippe Soubeyran¹

¹Aix-Marseille Univ, INSERM, CNRS,Institut Paoli-Calmettes, CRCM

Journal (Biochemistry)

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

James W. Saville¹, Luca A. Troman², Franck Duong Van Hoa¹

¹Department of Biochemistry,University of British Columbia, ²School of Biochemistry,University of Bristol

Journal (Biochemistry)

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Fernando Lopitz-Otsoa¹, Teresa C Delgado¹, Sofía Lachiondo-Ortega¹, Mikel Azkargorta², Felix Elortza², Manuel S Rodríguez³, María Luz Martínez-Chantar¹

¹Liver Disease and Liver Metabolism Lab,CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), ²Proteomics Platforms,CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, ³Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS,UbiCARE

Journal (Biochemistry)

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Alien Balian*^1,2,3, Javier Garcia Gonzalez*^1,2,3, Nora Bastida^1,3, Khadija-Tul Kubra Akhtar^1,3, Baris A. Borsa^1,2,3, Frank J. Hernandez^1,2,3

¹Department of Physics, Chemistry and Biology,Linköping University, ²Wallenberg Centre for Molecular Medicine (WCMM), ³Nucleic Acids Technologies Laboratory (NAT-lab),Linköping University

Journal (Biochemistry)

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Michael Vilkhovoy¹, David Dai¹, Sandra Vadhin*¹, Abhinav Adhikari*¹, Jeffrey D. Varner¹

¹Robert Frederick Smith School of Chemical and Biomolecular Engineering,Cornell University

Journal (Biochemistry)

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Fernanda Festa^1,2, Joshua Labaer¹

¹Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute,Arizona State University, ²Department of Pediatrics, and Biochemistry/Molecular Biology, College of Medicine,Pennsylvania State University

Journal (Biochemistry)

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

Ashley A. Martin^1,2, Simon Alford³, Janet E. Richmond¹

¹Department of Biological Sciences,University of Illinois at Chicago, ²Department of Integrative Biology and Physiology,University of Minnesota, ³Anatomi ve Hücre Biyolojisi Anabilim Dalı,University of Illinois at Chicago

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