JOVE JOURNAL

Biyokimya

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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Gatikrushna Singh*¹, Sarah M. Fritz*², Arnaz Ranji², Deepali Singh³, Kathleen Boris-Lawrie^1,2

¹Department of Veterinary & Biomedical Sciences,University of Minnesota, ²Department of Veterinary Biosciences,Ohio State University, ³School of Biotechnology,Gautam Buddha University

Journal (Biochemistry)

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Jonathan Fischer¹, Roger A. Johnson², Elizabeth Boon¹

¹Kimya Bölümü,Stony Brook University, ²Department of Physiology and Biophysics,Stony Brook University

Journal (Biochemistry)

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Masatake Kanai¹, Shoji Mano^1,2, Mikio Nishimura³

¹Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity,National Institute for Basic Biology, ²Department of Basic Biology,SOKENDAI (The Graduate University for Advanced Studies), ³Department of Cell Biology,National Institute for Basic Biology

Journal (Biochemistry)

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Ryan W. Clinton¹, Jason A. Mears¹

¹Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Journal (Biochemistry)

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Clemens Entzian¹, Thomas Schubert¹

¹2bind GmbH

Journal (Biochemistry)

A Rapid Laser Probing Method Facilitates the Non-invasive and Contact-free Determination of Leaf Thermal Properties

Johannes F. Buyel^1,2, Hannah M. Gruchow¹, Martin Wehner³

¹Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., ²Institute for Molecular Biotechnology,RWTH Aachen University, ³Fraunhofer Institute for Laser Technology ILT,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

Journal (Biochemistry)

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

Jakob V. Larsen¹, Claus M. Petersen¹

¹Department of Biomedicine,Aarhus University

Journal (Biochemistry)

Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins

Jenna K. Zalewski¹, Simone Heber^1,2, Joshua H. Mo¹, Keith O'Conor¹, Jeffrey D. Hildebrand¹, Andrew P. VanDemark¹

¹Department of Biological Sciences,University of Pittsburgh, ²Institute of Structural Biology,German Research Center for Environmental Health

Journal (Biochemistry)

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Martha E. Brennich¹, Adam R. Round^2,3, Stephanie Hutin⁴

¹Structural Biology Group,European Synchrotron Radiation Facility, ²European Molecular Biology Laboratory, ³School of Chemical and Physical Sciences,Keele University, ⁴Groupe de Microscopie Electronique et Méthodes,Institut de Biologie Structurale

Journal (Biochemistry)

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Christian Pichlo¹, Angelika A. Montada¹, Magdalena Schacherl¹, Ulrich Baumann¹

¹Institute of Biochemistry,University of Cologne

Journal (Biochemistry)

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Clément Danis*^1,2, Clément Despres*¹, Luiza M. Bessa¹, Idir Malki¹, Hamida Merzougui¹, Isabelle Huvent¹, Haoling Qi¹, Guy Lippens¹, François-Xavier Cantrelle¹, Robert Schneider¹, Xavier Hanoulle¹, Caroline Smet-Nocca¹, Isabelle Landrieu¹

¹UMR8576, CNRS,Lille University, ²UMR-S1172, INSERM CNRS,Lille University

Journal (Biochemistry)

Biomass Conversion to Produce Hydrocarbon Liquid Fuel Via Hot-vapor Filtered Fast Pyrolysis and Catalytic Hydrotreating

Huamin Wang¹, Douglas C. Elliott¹, Richard J. French², Steve Deutch², Kristiina Iisa²

¹Chemical and Biological Process Development,Pacific Northwest National Laboratory (PNNL), ²National Bioenergy Center,National Renewable Energy Laboratory (NREL)

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