Human Ovarian Epithelium Organoids: A Model for Tissue Repair and Drug Testing
1Department of Anatomy and Embryology,Leiden Üniversitesi Tıp Merkezi, 2The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),Leiden Üniversitesi Tıp Merkezi, 3Department of Obstetrics and Gynaecology,Amsterdam University Medical Center, 4Centre of Expertise on Gender Dysphoria,Amsterdam UMC, 5Amsterdam Reproduction and Development Research Institute, 6Department of Gynaecology,Leiden Üniversitesi Tıp Merkezi, 7Ghent-Fertility and Stem Cell Team (G-FAST), Department of Reproductive Medicine,Ghent University Hospital
Özet
This protocol describes establishing three-dimensional (3D) tissue organoids from primary human ovarian surface epithelium (hOSE) cells. The protocol includes isolation of hOSE from freshly collected ovaries, cellular expansion of the hOSE, cryopreservation-thawing procedures, and organoid derivation. Immunofluorescence, quantitative analysis, and showcasing utility as a screening platform are included.
