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Mitochondrial Fractionation in Small Tissue Samples for Supercomplex Analysis

Mitochondrial Fractionation in Small Tissue Samples for Supercomplex Analysis

May 03, 2024

Author Spotlight: Unveiling Oxidative Phosphorylation System Dynamics and Mitochondrial Roles in Health and Disease

Isolation of Enriched Mitochondrial Fractions from Cell Cultures and Small Animal Tissue Samples for Gel Activity Assays

Analyzing Mitochondrial Supercomplexes in Mammalian Tissue Fractions via In-Gel Activity Assays

Raquel Moreno-Loshuertos^1,2, Patricio Fernández-Silva^1,2

¹Department of Biochemistry,University of Zaragoza, ²Institute for Biocomputation and Physics of Complex Systems,University of Zaragoza

Özet

This protocol describes a technique for the analysis of respiratory supercomplexes when only small amounts of samples are available.

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