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Optimized Protocol for Retinal Organoid Sample Preparation for TEM

Optimized Protocol for Retinal Organoid Sample Preparation for TEM

June 07, 2024

制备用于透射电子显微镜的视网膜类器官样品

用于 TEM 样品制备的视网膜类器官固定和固定后程序

用于 TEM 样品制备的固定视网膜类器官的染色、脱水和浸润

用于透射电子显微镜的视网膜类器官包埋和半薄层定位

Xiaoqing Liu*^1,2, Bilin Rao*^1,2, Qingyang Lin^1,2, Meiling Gao^1,2, Jun Zhang^1,2

¹State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital,Wenzhou Medical University, ²Laboratory of Retinal Physiology and Disease, School of Ophthalmology and Optometry,Wenzhou Medical University

Özet

该协议为用于透射电子显微镜的视网膜类器官样品提供了优化和详细的制备程序。它适用于涉及成熟视网膜类器官中突触分析的应用。

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