Preparing Retinal Organoid Samples for Transmission Electron Microscopy

Xiaoqing Liu; Bilin Rao; Qingyang Lin; Meiling Gao; Jun Zhang

doi:10.3791/66590

Journal

Neurociência

Optimized Protocol for Retinal Organoid Sample Preparation for TEM

June 07, 2024

Author Spotlight: Analyzing the Synaptic Ultrastructure in Mature Retinal Organoids Using TEM

01:40 min

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216 Views

Retinal Organoid Fixation and Post Fixation Procedure for TEM Sample Preparation

01:02 min

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18 Views

Staining, Dewatering, and Infiltration of Fixed Retinal Organoids for TEM Sample Preparation

01:47 min

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10 Views

Retinal Organoid Embedding and Semi-Thin Slice Positioning for Transmission Electron Microscopy

01:18 min

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9 Views

Xiaoqing Liu*^1,2, Bilin Rao*^1,2, Qingyang Lin^1,2, Meiling Gao^1,2, Jun Zhang^1,2

¹State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital,Wenzhou Medical University, ²Laboratory of Retinal Physiology and Disease, School of Ophthalmology and Optometry,Wenzhou Medical University

Summary

This protocol provides an optimized and elaborate preparation procedure for retinal organoid samples for transmission electron microscopy. It is suitable for applications that involve the analysis of synapses in mature retinal organoids.