Real-time Evaluation of Absolute, Cytosolic, Free Ca<sup>2+</sup> and Corresponding Contractility in Isolated, Pressurized Lymph Vessels

Soumiya Pal; Ashim K. Bagchi; Amanda J. Stolarz

doi:10.3791/66535

Dergi

İmmünoloji ve Enfeksiyon

Real-Time Analysis of Calcium and Contractility in Isolated Lymph Vessels

March 22, 2024

Author Spotlight: Innovative Methods in Lymphedema and Hypertension Research

01:56 min

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404 Views

Setting Up the Perfusion Chamber to Measure Contractility in Isolated, Pressurized Lymph Vessels

00:45 min

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80 Views

Isolation and Cannulation of Mesenteric Lymph Vessels to Measure Cytosolic, Free Calcium and Vessel Diameter in Contracting Lymph Vessels

01:59 min

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100 Views

Measurement of the Absolute Concentrations of [Ca²⁺]_i in Isolated, Pressurized Lymph Vessels

04:19 min

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67 Views

Soumiya Pal¹, Ashim K. Bagchi¹, Amanda J. Stolarz¹

¹Department of Pharmaceutical Sciences, College of Pharmacy,University of Arkansas for Medical Sciences

Özet

This protocol describes a method to simultaneously measure cytosolic, free calcium [Ca²⁺]_i and vessel diameter in contracting lymph vessels in real time and then calculate absolute Ca²⁺ concentrations as well as contractility/rhythmicity parameters. This protocol can be used to study Ca²⁺ and contractile dynamics across a variety of experimental conditions.