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Quantifying NET Formation Using Dual-Dye Live Cell Analysis

Quantifying NET Formation Using Dual-Dye Live Cell Analysis

December 01, 2023

Author Spotlight: Quantifying Neutrophil Extracellular Traps in Disease and Drug Screening Using Dual-Color Live-Cell Imaging

02:01 min
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Human Neutrophil Staining and Preparation of Neutrophil Assay Plate for Live Cell Analysis

01:37 min
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Scanning Plate Setup and Quantitative Analysis of Neutrophil Extracellular Traps Formation in Human Neutrophils

03:49 min
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1Systemic Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health (NIH)

Özet

We present an automated high-throughput method to quantify neutrophil extracellular traps (NETs) utilizing the live cell analysis system, coupled with a membrane permeability-dependent dual-dye approach.

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