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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

November 10, 2023

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

FACS Enrichment of Zebrafish Gut Cell Suspension for scRNAseq

Naomi J. M. Kakiailatu¹, Laura E. Kuil^1,2, Eric Bindels³, Joke T. M. Zink³, Michael Vermeulen³, Veerle Melotte⁴, Maria M. Alves¹

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Özet

Here, we describe a method for gut isolation from zebrafish larvae at 5 days post fertilization, for single-cell RNA sequencing analysis.

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