Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Yi-Kai Liu; Zhuojun Luo; Anton Iliuk; Weiguo Andy Tao

doi:10.3791/65844

Dergi

Biyokimya

Enhancing EV Isolation for Precise Biofluid Analysis

October 27, 2023

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

01:49 min

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1,908 Views

Extracellular Vesicle (EV) Isolation from Human Urine Samples Using the EVtrap Approach

01:54 min

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211 Views

Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis

02:56 min

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192 Views

Proteome/Phosphoproteome Analysis of the Extracellular Vesicle (EV) Samples Using LC-MS/MS

02:58 min

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225 Views

Yi-Kai Liu¹, Zhuojun Luo¹, Anton Iliuk^1,2, Weiguo Andy Tao^1,2,3,4

¹Department of Biochemistry,Purdue Üniversitesi, ²Tymora Analytical Operations, ³Kimya Bölümü,Purdue Üniversitesi, ⁴Purdue Institute for Cancer Research,Purdue Üniversitesi

Özet

The present protocol provides detailed descriptions for the efficient isolation of urinary extracellular vesicles utilizing functionalized magnetic beads. Moreover, it encompasses subsequent analyses, including western blotting, proteomics, and phosphoproteomics.