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Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

August 25, 2023

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

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Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

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Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion

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1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, 2Department of Biochemistry, Faculty of Medicine and Dentistry,Alberta Üniversitesi

Özet

This protocol describes a setup for the crystallization of the sterol transporter ABCG5/G8. ABCG5/G8 is reconstituted into bicelles for hanging-drop crystallization. The protocol does not require specialized materials or substrates, making it accessible and easy to adapt in any laboratory for determining the protein structure through X-ray crystallography.

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