Modeling Multiple Sclerosis in the Two Sexes: MOG<sub>35-55</sub>-Induced Experimental Autoimmune Encephalomyelitis

Brigitta Bonaldo; Antonino Casile; Francesca Montarolo; Antonio Bertolotto

doi:10.3791/65778

Dergi

Nörobilim

Induction and Evaluation of Experimental Autoimmune Encephalomyelitis in Mouse

October 13, 2023

Author Spotlight: Creating a Versatile Experimental Autoimmune Encephalomyelitis Model Relevant for Both Male and Female Mice

02:08 min

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Preparation of MOG35-55 Emulsion for Immunization of Mouse

01:01 min

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Immunization of Mouse with MOG35-55 to Induce Experimental Autoimmune Encephalomyelitis

00:56 min

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Clinical Score and Motor Performance Evaluation of Mouse Induced with Experimental Autoimmune Encephalomyelitis

01:52 min

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Brigitta Bonaldo^1,2, Antonino Casile^1,2,3, Francesca Montarolo^1,2, Antonio Bertolotto^1,4

¹Neuroscience Institute Cavalieri Ottolenghi (NICO), ²Department of Neuroscience “Rita Levi-Montalcini”,University of Turin, ³School of Pharmacy, Pharmacology Unit,University of Camerino, ⁴Koelliker Hospital

Özet

Experimental autoimmune encephalomyelitis is one of the most widely used murine models of multiple sclerosis. In the current protocol, C57BL/6J mice of both sexes are immunized with myelin oligodendrocyte glycoprotein peptide, resulting mainly in ascending paresis of the tail and limbs. Here we discuss the protocol of EAE induction and evaluation.