Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina

Ryan  M. Hecht; Qing Shi; Tavita R. Garrett; Benjamin Sivyer; Catherine Morgans

doi:10.3791/65757

Studying Bipolar and Horizontal Neuronal Cells in the Murine Split Retina

January 16, 2024

Author Spotlight: Using the Split Retina Technique for Enhanced Access and Accelerated Experiments

01:44 min

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Mouse Retinal Dissection for Split Retina Application

02:00 min

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Splitting of Murine Retina for Immunofluorescence Analysis

01:52 min

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Mouse Split Retina Preparation for Immunofluorescence Analysis

02:51 min

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Ryan M. Hecht, Qing Shi, Tavita R. Garrett, Benjamin Sivyer, Catherine Morgans

¹Department of Chemical Physiology and Biochemistry,Oregon Health and Science University, ²Casey Eye Institute,Oregon Health and Science University

Özet

This work presents an alternative flatmount retina preparation in which the removal of photoreceptor cell bodies enables faster antibody diffusion and improved patch pipette access to inner retinal neurons for immunohistochemistry, in situ hybridization, and electrophysiology experiments.