Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model

Athar Shadmani; Hala Shakib Dhowre; Ozlem Ercal; Xiang Qi Meng; Albert Y. Wu

doi:10.3791/65609

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A Modified Biopsy Punch Method for Precise Alkali Burns in Mice

August 04, 2023

Author Spotlight: Advancing Research in Corneal Opacity Treatment and Regeneration

02:00 min

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Inducing Corneal and Limbal Alkali Injury in a Mouse Model

05:42 min

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Athar Shadmani¹, Hala Shakib Dhowre¹, Ozlem Ercal¹, Xiang Qi Meng², Albert Y. Wu¹

¹Department of Ophthalmology,Stanford University School of Medicine, ²McGill University Faculty of Medicine and Health Sciences

Özet

This protocol describes a method to induce an accurate and reproducible corneal and limbal alkali injury in a mouse model. The protocol is advantageous as it allows for an evenly distributed injury to the highly curved mouse cornea and limbus.

A Modified Biopsy Punch Method for Precise Alkali Burns in Mice

Author Spotlight: Advancing Research in Corneal Opacity Treatment and Regeneration

Inducing Corneal and Limbal Alkali Injury in a Mouse Model

Özet

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