A Set of Screening Techniques for a Quick Overview of the Neutrophil Function

Isabelle Souza Luz; Raquel Takaya; Daiane Gonzaga Ribeiro; Natanael Sales Silva; Lucas Fontes; Mariana S. Castro; Wagner Fontes

doi:10.3791/65329

Dergi

İmmünoloji ve Enfeksiyon

Neutrophil Functional Assays for Screening Different Signaling Pathways

February 09, 2024

Author Spotlight: Balancing Speed, Cost, and Accuracy in Neutrophil Function Assessment with NeutroFun Screen Protocol

02:02 min

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Neutrophil Isolation and Activation

02:54 min

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Nitrotetrazolium Blue Chloride (NBT) Assay for Evaluating ROS Production

02:59 min

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Neutrophil Phagocytosis Assay

01:58 min

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Real-Time Polymorphonuclear Neutrophils (PMN) Chemotaxis Assay

01:26 min

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Neutrophil Extracellular Trap (NET) Suggestive Assay

01:22 min

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Isabelle Souza Luz¹, Raquel Takaya¹, Daiane Gonzaga Ribeiro¹, Natanael Sales Silva¹, Lucas Fontes², Mariana S. Castro¹, Wagner Fontes¹

¹Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology,University of Brasilia, ²Institute of Physics,University of Brasilia

Özet

This protocol features a set of neutrophil functional assays to be used as a screening method to cover functions from different signaling pathways. The protocol includes an initial and simple evaluation of cell viability, purity, reactive oxygen species production, real-time migration, phagocytosis, and a preliminary suggestion of neutrophil extracellular traps.