Source: Greimers, L., et al. Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description. J. Vis. Exp. (2018).
This video demonstrates a technique for injecting platelet-rich plasma (PRP) into the sectioned Achilles tendon of rats. A small segment is excised from the Achilles tendon of a recipient rat, the site is sutured, and the rat is allowed to recover. Concurrently, whole blood is collected from a donor rat and centrifuged to obtain PRP, which is then activated and injected into the sutured site of the recipient rat to induce tendon repair.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Animal Preparation
2. Surgical Procedure
3. PRP Preparation
Figure 1: Experimental design of the study.
Figure 2: Surgical Procedure.
(a) The tendon complex after removal of the surrounding fascia.
(b) Removal of the plantaris tendon.
(c) Removal of a section of the Achilles tendon.
(d) The 2 tendons that have been removed.
The authors have nothing to disclose.
Xylazine (Xyl-M) | VMD | none | anesthetic |
Ketamin (Jétamine 1000 CEVA) | CEVA Santé Animale | none | anesthetic |
Buprenorphin (Vetergésic Multidosis) | ALSTOE | none | Painkiller |
iso-Betadine | MEDA-Pharma | none | Desinfectant |
Resorbable yarn Vicryl 6/0 | Johnson & Johnson | ||
Nembutal | CEVA Santé Animale | none | Anesthetic |
Paraformaldehyde | Sigma-Aldrich | P6148 | Preserves structure of the tissue |
Isopropanol 100% | VWR | 2,09,22,364 | |
Ethanol 95% | VWR | 2,08,23,362 | |
Xylene | VWR | 28973.363 | |
Paraffin | VWR | LEIC3950.1006 | |
Hematoxylin | Millipore | 1.15938.0025 | Colorant |
Eosin | Millipore | 1.15935.0100 | Colorant |
Eukitt | Sigma-Aldrich | 3989 | Mounting Medium |
CaCl2 |
.