Source: Trevaskis, N. L. et al., The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport. J. Vis. Exp. (2015)
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparations the Day Before the Surgical Procedure
2. Preparations Immediately Prior to Commencing the Surgical Procedure
3. Cannulation of the Duodenum
Figure 1. Diagrammatic representation of the shape of the beveled tip of a carotid artery or lymph cannula, and the J-shaped beveled tip of a duodenal cannula.
Figure 2. Photographs of (A) the abdomen shaved in preparation to cannulate the lymph duct, (B) the abdominal muscle wall opened with a straight 4 cm incision extending from the midline to the right flank to access the lymph duct, and (C) the superior mesenteric lymph duct (in yellow circle) perpendicular to the right kidney.
The authors have nothing to disclose.
Sterile saline | Baxter healthcare | AHB 1307 | Any brand can be used. Example here is Baxter 100 ml saline bags, box of 50 |
70 % ethanol in water | Any | Any brand can be used | |
Chlorhexidine gluconate solution (Microshield 4) | Livingstone International | JJ60243L | Any brand can be used. http://www.livingstone.com.au/?PG=search_result&CAT=6&search |
Betadine solution | Livingstone International | BU0510 | Any brand can be used. http://www.livingstone.com.au/?PG=search_result&CAT=6&search |
Ilium Ketamil (Ketamine 100 mg/ml) | PROVET VICTORIA | KETA I 1 | http://www.provet.com.au/ |
Ilium Xylazil (Xylazine 100 mg/ml) | PROVET VICTORIA | TRO-3828 | http://www.provet.com.au/ |
ACP 10 Injection (Acepromazine 10 mg/ml) | PROVET VICTORIA | VTG-DACP010020 | http://www.provet.com.au/ |
Heparin (35000I.U. in 35 mL) | Sigma Pharmaceuticals | 337220 | http://sigmaco.com.au/ |
Ethylenediaminetetraacetic acid (EDTA) disodium salt dihydrate | Sigma-Aldrich | E1644 | Any brand can be used. Example here is disodium salt of EDTA from Sigma. |
Polyethylene (PE) cannula o.d. 0.96 mm x i.d. 0.58 mm | Microtube extensions | PE8050 | Any brand can be used. Example here is PE tubing 0.8×0.5 mm, 30 m |
Polyethylene (PE) cannula o.d. 0.8 mm x i.d. 0.5 mm | Microtube extensions | PE9658 | Any brand can be used. Example here is PE tubing 0.96×0.58 mm, 30 m |
Ruler | Any | Any brand can be used | |
Markers | Any | Any brand can be used | |
Cigarette lighter | Any | Any brand can be used | |
Cyanoacrylate glue | Any | Any brand can be used | |
23 gauge needles | Livingstone International | DN23GX0.75LV | Any brand can be used. Example here is Livingstone Disposable Needle, Sterile, 23GX0.75inch, 100/BOX. http://www.livingstone.com.au/?PG=search_result&CAT=6&search =DN23GX0.75LV |
25 gauge needles | Livingstone International | DN25GX1.0LV | Any brand can be used. Example here is Livingstone Disposable Needle, Sterile, 25GX1.0inch, 100/BOX. http://www.livingstone.com.au/?PG=search_result&CAT=6&search =DN25GX1.0LV |
1 ml syringe | Livingstone International | T3SS01TA | Any brand can be used. Example here is Terumo syringe 1 ml Slip Tuberculin 100/Box. http://www.livingstone.com.au/?PG=search_result&CAT=6&search =T3SS01TA |
10 ml syringe | Livingstone International | T3SS10SA | Any brand can be used. Example here is Terumo syringe 10 ml Slip 100/Box. http://www.livingstone.com.au/?PG=search_result&CAT=6&search =T3SS10SA |
Gauze swabs | Livingstone International | GSC075 | Any brand can be used and cut to required size. Example here is gauze swabs cotton filled 7.5×7.5 cm, 8 ply. http://www.livingstone.com.au/?PG=search_result&CAT=6&search =GSC075 |
Heating pad | Ratek | WT1 | Any brand that keeps temperature at 37C can be used. Example here is Ratek warming tray. |
Surgical light | Harvard Apparatus | 72-0215 with 72-0267 | Any brand can be used. Example here is Harvard apparatus V-Lux 1000 Cold Light Source with Bifurcated Gooseneck Light Guide, Black, 4.7 mm fiber diameter (each arm). http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_50601_ |
Surgical microscope | Zeiss | 495005-0014-000 | Any brand can be used. Example here is Zeiss Stereomicroscope Stemi 2000-C with Stand S Double Spot and KL 300 LED. https://www.micro-shop.zeiss.com/?l=en&p=us&f=e&i=10143 |
Silk suture | Livingstone International | DTSK163019F4 | Any brand can be used. Example here is * Email this item to my friend 3/8 Circle Reverse Cut Silk Suture 3/0 Thread 19mm. http://www.livingstone.com.au/?PG=search_result&CAT=6&search =DTSK163019F4 |
Scalpel blades | Fine Science Tools (FST) | 10020-00 | Any brand can be used. Example here is FST Scalpel Blade #20. http://www.finescience.ca/Special-Pages/Products.aspx?ProductId=191 |
Scalpel handle | Fine Science Tools (FST) | 10004-13 | Any brand can be used. Example here is FST Scalpel Handle #4. http://www.finescience.ca/Special-Pages/Products.aspx?ProductId=298&CategoryId=51 |
1 x Small surgical scissors | Fine Science Tools (FST) | 14060-09 | Any brand can be used. Example here is FST Fine Scissors, 9 cm with 21 mm cutting edge, sharp, straight. http://www.finescience.ca/Special-Pages/Products.aspx?ProductId=40&CategoryId=17 |
2 x Forceps with serrated curved tip | Fine Science Tools (FST) | 11001-13 | Any brand can be used. Example here is FST 13 cm standard pattern forceps with curved 2.8×1.4 mm tip. http://www.finescience.ca/Special-Pages/Products.aspx?ProductId=405&CategoryId=32 |
2 x Hemostats | Fine Science Tools (FST) | 13010-12 | Any brand can be used. Not all operators use the hemostats. Example here is FST 12 cm Micro-Mosquito Hemostats with 20 mm length x 1.3 mm width serrated, straight tip. http://www.finescience.ca/Special-Pages/Products.aspx?ProductId=377&CategoryId=33 |
1 x Suture needle holder | Fine Science Tools (FST) | 12001-13 | Any brand can be used. Example here is FST 13cm Hasley Needle Holder with 16 mm length x 1.9 mm width tip. http://www.finescience.ca/Special-Pages/Products.aspx?ProductId=254&CategoryId=70 |