Bipolar Radiofrequency-Based Hemorrhage Arrest in Solid Organ Injury: A Technique to Arrest Bleeding in Porcine Model With Liver Injury Using Electrosurgery
Published: April 30, 2023
Abstract
Source: Clements, T. W., et al. A Saline/Bipolar Radiofrequency Energy Device As an Adjunct for Hemostasis in Solid Organ Injury/Trauma. J. Vis. Exp. (2020).
This video demonstrates a technique of bipolar radiofrequency energy-based transcollation for hemorrhage arrest in solid organ injury in a porcine model. This method is helpful for the surgical management of traumatic injury by achieving hemostasis by arresting bleeding at the injury site.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Model Preparation House the 50 kg adult male pig in an animal care facility for 1 week prior to the surgery to acclimatize the animal to the housing conditions and the handlers. Fast the model for a minimum of 6 h prior to the initiation of anesthesia. Anesthetize the model using an intramuscular injection of ketamine (33 mg/k…
Representative Results
Figure 1: Saline/bipolar radiofrequency (SBRF) energy device. (A) This panel shows the SBRF device handpiece with the single-button design. (B) This panel shows the SBRF's 6.0 blunt bipolar sealing tip.
Disclosures
The authors have nothing to disclose.
Materials
| Aquamantys pump generator | Medtronic | 40-402-1 | |
| Aquamantys 6.0 bipolar sealer | Medtronic | 23-112-1 | |
| Electrosurgical pencil with tip | Megadyne | 39 | |
| Porcine animal | |||
| Porcine ventilator/induction and anesthetic medications | |||
| 2 x 1 liter bags of 0.9% normal saline | |||
| 2 x scalpels (#10) | |||
| Belfour abdominal retractor | |||
| Suction tubing | |||
| Suction tip | |||
| Suction device/wall connector | |||
| Suction canister | |||
| Debakey forceps | |||
| Metz scissors | |||
| Curved Mayo scissors | |||
| Closing suture (1-0 Nylon) | |||
| 20 x Laparotomy sponges | |||
| 2 x Kelley clamps | |||
| 2 x snap clamps |
Tags
Cite This Article
Bipolar Radiofrequency-Based Hemorrhage Arrest in Solid Organ Injury: A Technique to Arrest Bleeding in Porcine Model With Liver Injury Using Electrosurgery. J. Vis. Exp. (Pending Publication), e20916, doi: (2023).