Source: Clements, T. W., et al. A Saline/Bipolar Radiofrequency Energy Device As an Adjunct for Hemostasis in Solid Organ Injury/Trauma. J. Vis. Exp. (2020).
This video demonstrates a technique of bipolar radiofrequency energy-based transcollation for hemorrhage arrest in solid organ injury in a porcine model. This method is helpful for the surgical management of traumatic injury by achieving hemostasis by arresting bleeding at the injury site.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Model Preparation
2. Device Preparation
3. Surgery: Laparotomy
4. Surgery: Simulated Solid Organ Injury
NOTE: The injuries described below represent a worsening hierarchy of injuries. The injuries are created by an expert trauma surgeon and hemostasis will be obtained by another surgeon.
5. Hemostasis
6. Sealing Small to Medium Bile Ducts
Figure 1: Saline/bipolar radiofrequency (SBRF) energy device. (A) This panel shows the SBRF device handpiece with the single-button design. (B) This panel shows the SBRF's 6.0 blunt bipolar sealing tip.
The authors have nothing to disclose.
Aquamantys pump generator | Medtronic | 40-402-1 | |
Aquamantys 6.0 bipolar sealer | Medtronic | 23-112-1 | |
Electrosurgical pencil with tip | Megadyne | 39 | |
Porcine animal | |||
Porcine ventilator/induction and anesthetic medications | |||
2 x 1 liter bags of 0.9% normal saline | |||
2 x scalpels (#10) | |||
Belfour abdominal retractor | |||
Suction tubing | |||
Suction tip | |||
Suction device/wall connector | |||
Suction canister | |||
Debakey forceps | |||
Metz scissors | |||
Curved Mayo scissors | |||
Closing suture (1-0 Nylon) | |||
20 x Laparotomy sponges | |||
2 x Kelley clamps | |||
2 x snap clamps |