Source: Boice, E. N., Snider, E. J. Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance. J. Vis. Exp. (2021).
In this video, we demonstrate the culturing method of a cornea-bearing anterior eye segment from a porcine eye. This culture system serves as an experimental model to study eye injuries.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Setting up anterior segments in organ culture dishes
2. Starting anterior segment organ culture
3. Daily maintenance of ASOC
The authors have nothing to disclose.
10-32 Polycarbonate straight plug, male threaded pipe connector | McMaster-Carr | 51525K431 | |
10-32 Socket cap screw, ½" | McMaster-Carr | 92196A269 | |
10 mL syringe | BD | 302995 | |
20 mL syringe | BD | 302830 | |
Anti-Anti | Gibco | 15240-096 | |
Ball-End L key | McMaster-Carr | 5020A25 | |
BD Intramedic PE 160 Tubing | Fisher Scientific | 14-170-12E | |
Cotton swabs | Puritan | 25-8061WC | |
DMEM media | ATCC | 30-2002 | |
FBS | ATCC | 30-2020 | |
Fine forceps | World Precision Instruments | 15914 | |
Glutamax | Gibco | 35050-061 | |
High temperature silicone O-ring, 2 mm wide, 4 mm ID | McMaster-Carr | 5233T47 | |
Nail (puncture object) | McMaster-Carr | 97808A503 | |
Nylon syringe filters | Fisher | 09-719C | |
PBS | Gibco | 10010-023 | |
Polycarbonate, three-way, stopcock with male luer lock | Fisher | NC9593742 | |
Stainless steel 18 G 90 degree angle dispensing needle | McMaster-Carr | 75165A81 | |
Stainless steel 18 G straight ½'' dispensing needle | McMaster-Carr | 75165A675 |