Chemical-induced Two-stage Skin Carcinogenesis Model: An Experimental In Vivo Mouse Model of Skin Cancer
Published: April 30, 2023
Abstract
Source: Vähätupa, M. et al. Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA). J. Vis. Exp. (2019)
This video describes the procedure to induce two-stage skin carcinogenesis to study tumor initiation by topical application of chemicals. In the first step, a mutagen, DMBA, is applied onto the skin of a mouse model which initiates tumor formation by causing mutations in the stem cells, followed by the application of a growth stimulator, TPA, which accelerates skin papilloma formation.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Experimental Animals, Reagents, and Equipment Use age and sex-matched mice. Start the study at 7-9 weeks of age because the skin in most mice is in telogen (the resting phase) around that age. Observe the behavior of the animals during the study and if they fight, which often happens with males, house them sep…
Disclosures
The authors have nothing to disclose.
Materials
| 7,12-Dimethylbenz[a]anthracene (DMBA) | Sigma | D3254-100MG | Harmful if swallowed and may cause cancer. Store protected from light. |
| Acetone | Sigma | 1000141011 | Evaporates rapidly and is inflammable. |
| Attane vet 1000 mg/g | Piramal Critical Care Limited | Liquid isoflurane for inhalation | |
| Battery-Operated Clipper Isis | Albert Kerlb GmbH | GT421 | For shaving the fur |
| CONTRAfluran-Restgasfilter | ZeoSys GmbH | For anesthesia | |
| Linex Nature N1030 Ruler 30 cm | Staples Business Advantage | 60383 | For measuring papillomas |
| Tacta pipette 100-1000 ul | Sartorius | LH-729070 | |
| Tacta pipette 20-200 ul | Sartorius | LH-729060 | |
| Phorbol 12-myristate 13-acetate aka 12-Otetradecanoylphorbol-13- acetate (TPA) | Enzo | BML-PE160-0001 | |
| Mekasoft | Mekalasi | 23008 | Table cover |
| Precision balance PLJ-C/PLJ-G | KERN & SOHN GmbH | PLJ 600-3CM | |
| Mice (Balb/c JRj) | Janvier labs | Other strains also possible | |
| Mice (C57BL/6JRj) | Janvier labs | Other strains also possible | |
| Panasonic Lumix DMC-FS5 Digital Camera | Panasonic |
Cite This Article
Chemical-induced Two-stage Skin Carcinogenesis Model: An Experimental In Vivo Mouse Model of Skin Cancer. J. Vis. Exp. (Pending Publication), e20356, doi: (2023).