India Ink Inflation: A Staining Method to Visualize Tumor Nodules
Published: April 30, 2023
Abstract
Source: Paschall, A. V. et al. An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis. J. Vis. Exp. (2016).
This video describes india ink inflation–a staining method–to quantify tumor nodules in tumor-bearing mouse lungs. After staining, the lungs are fixed in Fekete's solution to observe tumor nodules in the lungs.
Protocol
1. Validation of Lung Metastases Using India Ink Inflation of Tumor-bearing Lungs NOTE: To validate the luciferase live tumor imaging results, perform India ink inflation of tumor-bearing mouse lungs to quantify tumor nodules. 4T1 cells also metastasize to other organs and tissues (Figure 1) and therefore, histological examination of these organs to validate tumor metastasis should be performed if needed. This protocol use lung metastasis as a e…
Representative Results
Figure 1. Visualization of Lung Tumor Nodules by India Ink Inflation. The lung of the tumor-bearing mouse was inflated with India ink and fixed in Fekete's solution. The white dots are lung metastases.
Disclosures
The authors have nothing to disclose.
Materials
| 9 mm AutoClip Applier | Becton Dickinson Primary Care Diagnostics. Sparks, MD | 427630 | |
| Sharp-Pointed Dissecting Scissors | Fisher | 8940 | |
| Dissecting Fine-Pointed Forceps | Fisher | 8875 | |
| 70% Ethanol | Ultrapure-usa.com | ||
| 1/2 CC 27 G 1/2 tuberculin syringe | Becton Dickinson and Co. NJ | 305620 |
Cite This Article
India Ink Inflation: A Staining Method to Visualize Tumor Nodules. J. Vis. Exp. (Pending Publication), e20222, doi: (2023).