The article describes a protocol to generate zebrafish embryos through natural mating and sorting their developmental stages. The protocol is used further for analysis of pancreatic β-cells by using transgenic fishes.
Protocol
1. Generating embryos through natural mating Culture embryos to 3 months of age, reproductive maturity. Segregate adult male and female fish from the desired strain into divided mating tanks on the evening before embryo collection, and add 2 males and 3 females to each tank. NOTE: Use of the transgenic insulin2a:mCherry fluorescent reporter strain allowed the analysis of pancreatic β-cells. Transfer fish to the …