Larval Locomotion Assay: High-Throughput Method to Study Neurobehavioral Toxicity of a Compound in Zebrafish Larvae
Published: April 30, 2023
Abstract
Source: Zhang B., et al. Studying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae. J. Vis. Exp. (2020)
This article describes zebrafish’s behavioral response to toxic compounds by monitoring larval locomotion. The protocol further looks at an example of zebrafish larvae’s response to environmental pollutant.
Protocol
1. Preparation for the behavior test Transfer 800 µL of exposure solution into every well of the 48 well microplate. NOTE: Use 16 wells for every group (i.e., the control solution, 5 µg/L, and 50 µg/L group). Use a 1 mL pipette tip to transfer 200 µL of exposure solution with one larva from the glass Petri dish into one well of the 48 well microplate. Transfer 4 mL of exposure solution into every well of the 6 well microplate.<br…
Materials
| 48-well-microplate | Corning | 3548 | Embyros housing |
| 6-well-microplate | Corning | 3471 | Embyros housing |
| Zebrabox | Viewpoint | ZebraBox | Behavior instrument |
| ZebraLab | Viewpoint | ZebraLab | Behavior software |
| Pipette | Eppendorf | 3120000267 | Transfer solution |
Cite This Article
Larval Locomotion Assay: High-Throughput Method to Study Neurobehavioral Toxicity of a Compound in Zebrafish Larvae. J. Vis. Exp. (Pending Publication), e20180, doi: (2023).