Calcium Imaging: A Method to Visualize Neural Activity in Live C. elegans
Published: April 30, 2023
Abstract
Source: Turek, M. et al. Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans. J. Vis. Exp. (2015).
This video introduces the concepts behind calcium imaging and includes an example protocol for time-lapse imaging of worms contained in molded agarose wells.
Protocol
This protocol is an excerpt from Turek et al, Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans, J. Vis. Exp. (2015). 1. Calcium Imaging Use transgenic strains expressing genetically encoded calcium sensors such as HBR16 (goeIs5[pnmr-1::SL1-GCaMP3.35-SL2::unc-54-3'UT…
Materials
| LED system | CoolLED | pE-2 | |
| Compound microscope | Nikon | TiE | |
| EMCCD camera | Andor | iXon 897 | now sold as iXon Ultra 897 |
| Z-stage for the microscope | Prior | Nano Scan Z | |
| X-Y stage for the microscope | Prior | Proscan III | |
| Red light filter | Chroma | ET660/50m | |
| 35 mm Petri dish | Falcon | Falcon Disposable Petri Dishes, Sterile, Corning |
Cite This Article
Calcium Imaging: A Method to Visualize Neural Activity in Live C. elegans. J. Vis. Exp. (Pending Publication), e20164, doi: (2023).