Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans
Published: April 30, 2023
Abstract
Source: Lee, S. Y. and Kang, K. Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans. J. Vis. Exp. (2017).
C. elegans egg-laying behavior can provide important information in developmental, reproductive, and anatomical studies. This video introduces the important steps of an egg laying assay and shows a sample experiment in which a drug is tested for reproductive toxicity.
Protocol
Lee and Kang, Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans, J. Vis. Exp. (2017). 1. C. elegans Egg Laying Assay Incubate the age-synchronized eggs on the NGM plate containing DMSO or etoposide for 64 h (before first birth) until the young adult sta…
Materials
| Caenorhabditis elegans N2 | Caenorhabditis Genetics Center (CGC) | Wild type | |
| Dimethyl sulfoxide | Sigma, USA | D2650 | |
| Escherichia coli OP50 | Caenorhabditis Genetics Center (CGC) | ||
| Etoposide | Sigma, USA | E1383 | |
| 35 × 10 mm Petri dish | SPL Life Sciences, South Korea | 10035 | |
| Stereo microscope | Nikon, Japan | SMZ800N |
Cite This Article
Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans. J. Vis. Exp. (Pending Publication), e20157, doi: (2023).