Fly Climbing Assay: A Method to Test General Locomotion in Drosophila melanogaster
Published: April 30, 2023
Abstract
Source: Gevedon, O., et al. In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster. J. Vis. Exp. (2019).
This video describes a simple method to quantify fly locomotion and shows the climbing assay performed. The fly climbing assay is a general test of motor function which relies on a negative geotaxis response.
Protocol
This protocol is an excerpt from Gevedon et al., In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster, J. Vis. Exp. (2019). 1. Climbing Assay (Adapted from Ali et al., J. Vis. Exp. (2011)) Validate the climbing assay. In this study, test the climbing assay based on previously described methodology by Ali et al., using…
Materials
| VWR® Drosophila Vial; Narrow | VWR | 75813-160 | |
| VWR® General-Purpose Laboratory Labeling Tape | VWR | 89097-912 | |
| Standard mouse pad | |||
| Stereoscope | Motic | Model SMZ-168 | |
| Drosophila Incubator | VWR | 89510-750 | |
| R software package |
Cite This Article
Fly Climbing Assay: A Method to Test General Locomotion in Drosophila melanogaster. J. Vis. Exp. (Pending Publication), e20078, doi: (2023).