Vacuolar or V-type pumps are a type of ATP-driven pumps mainly present on the membranes of eukaryotic subcellular compartments like plant vacuoles, lysosomes, and endosomes. V-type pumps are turbine-like structures with two domains— V1 and V0. The transmembrane V0 domain comprises multiple subunits, including an a-subunit and a ring of membrane-spanning c-subunits. The cytosolic V1 domain is also made up of numerous subunits, including a hexamer of alternating A and B subunits, a rotor, and peripheral stators. Consider a V-type pump present on the lysosomal membrane of a eukaryotic cell. As a molecule of ATP enters the hexameric subunit of the V1 domain, it gets hydrolyzed into ADP and inorganic phosphate. The energy released from ATP hydrolysis rotates the central stalk and the c-ring subunits of the V0 domain. A proton from the cytosol enters through a channel in the a-subunit and binds to a c-ring subunit. Simultaneously, a lumen-facing channel of the a-subunit releases the proton into the lysosome making space for another proton to bind the c-subunit from the cytosol.