JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Roleen Sharma^1,2, Kim M. O'Sullivan², Stephen R. Holdsworth², Philip G. Bardin^1,2, Paul T. King^1,2

¹Monash Lung and Sleep,Monash Medical Centre, ²Monash University

Journal (Immunology and Infection)

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Thomas A. Angelovich^1,2, Anna C. Hearps^1,3, Anna Maisa¹, Theodoros Kelesidis⁴, Anthony Jaworowski^1,3

¹Centre for Biomedical Research,Burnet Institute, ²School of Health and Biomedical Sciences,RMIT University, ³Department of Infectious Diseases,Monash University, ⁴University of California, Los Angeles

Journal (Immunology and Infection)

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Praveen Kumar Balne^1,2, Veonice Bijin AU³, Louis Tong², Arkasubhra Ghosh⁴, Mukesh Agrawal⁵, John Connolly³, Rupesh Agrawal¹

¹National Healthcare Group Eye Institute,Tan Tock Seng Hospital, ²Singapore Eye Research Institute (SERI), ³Institute of Molecular and Cell Biology,A*STAR, ⁴GROW Research Laboratory,Narayana Nethralaya Foundation, ⁵Vimta Labs Limited

Journal (Immunology and Infection)

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Shubhendu Sen Roy¹, Huy Cong Xuan Nguyen¹, Thomas A. Angelovich^2,3, Anna C. Hearps², Diana Huynh^1,4, Anthony Jaworowski^2,4, Theodoros Kelesidis¹

¹University of California, Los Angeles, ²Centre for Biomedical Research,Burnet Institute, ³School of Health and Biomedical Sciences,RMIT University, ⁴Department of Infectious Diseases,Monash University

Journal (Immunology and Infection)

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

Alessandra M. Agostinho Hunt¹, Jacob A. Gibson¹, Casandra L. Larrivee¹, Sandra O'Reilly², Svetlana Navitskaya², Julia V. Busik², Christopher M. Waters¹

¹Department of Microbiology and Molecular Genetics,Michigan State University, ²Department of Physiology,Michigan State University

Journal (Immunology and Infection)

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Yoo Jung Choi*¹, Jinhui Zhu*¹, Steve Chung*¹, Naveed Siddiqui*¹, Qingping Feng¹, Peter B. Stathopulos¹

¹Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario

Journal (Immunology and Infection)

Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis

Jesse Q. Nguyen¹, Xhavit Zogaj¹, Aanuoluwa A. Adelani¹, Ping Chu², Jieh-Juen Yu¹, Bernard P. Arulanandam¹, Karl E. Klose¹

¹South Texas Center for Emerging Infectious Diseases,University of Texas San Antonio, ²Midwestern University

Journal (Immunology and Infection)

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Yo Ching Cheng*¹, Samia Hannaoui*¹, Theodore Ralph John², Sandor Dudas³, Stefanie Czub³, Sabine Gilch¹

¹Dept. of Ecosystem and Public Health, Calgary Prion Research Units, Faculty of Veterinary Medicine,University of Calgary, ²Dept. of Molecular Biology,University of Wyoming, ³Canadian Food Inspection Agency,Lethbridge Laboratories

Journal (Immunology and Infection)

Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment

Rafael Taeho Han¹, Seung Keun Back², JaeHee Lee¹, Hye young Kim¹, Hee Jin Kim³, Heung Sik Na¹

¹Neuroscience Research Institute & Department of Physiology, College of Medicine,Korea University, ²Department of Pharmaceutics and Biotechnology, College of Medical Engineering,Konyang University, ³Division of Biological Science and Technology, Science and Technology College,Yonsei University Wonju Campus

Journal (Immunology and Infection)

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Nikki E. Freed¹

¹Institute of Natural and Mathematical Sciences,Massey University

Journal (Immunology and Infection)

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Shengbin Li¹, Gwantwa Mwakalundwa¹, Pamela J. Skinner¹

¹Department of Veterinary and Biomedical Sciences,University of Minnesota

Journal (Immunology and Infection)

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Helia N. Sanchez¹, Tian Shen^1,2, Dawn Garcia^1,3, Zhao Lai^1,3, Paolo Casali¹, Hong Zan¹

¹Department of Microbiology, Immunology and Molecular Genetics,University of Texas Long School of Medicine at San Antonio, ²Xiangya School of Medicine,Cental South University, ³Greehey Children’s Cancer Research Institute,University of Texas Long School of Medicine at San Antonio

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