JOVE JOURNAL

Developmental Biology

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Journal (Developmental Biology)

Formation of Human Thymus Organoids in Three-Dimensional Fibrin Hydrogels

Manon d'Arco*¹, Nathan Provin*¹, Pierre Maminirina², Olivier Baron², Carole Guillonneau¹, Laurent David^1,3, Matthieu Giraud¹

¹Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ²CHU Nantes, Nantes Université, Department of Pediatric and Congenital Cardiac Surgery, ³Nantes Université, CHU Nantes, INSERM, CNRS, BioCore, SFR Bonamy

Journal (Developmental Biology)

3D Human Thymic Organoid Model Preparation for Studying T-Cell Biology

Manon d'Arco*¹, Nathan Provin*¹, Pierre Maminirina², Olivier Baron², Carole Guillonneau¹, Laurent David^1,3, Matthieu Giraud¹

¹Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ²CHU Nantes, Nantes Université, Department of Pediatric and Congenital Cardiac Surgery, ³Nantes Université, CHU Nantes, INSERM, CNRS, BioCore, SFR Bonamy

Journal (Developmental Biology)

Rapid Isolation of Stage I Oocytes in Zebrafish Devoid of Granulosa Cells

Qianwen Zheng¹, Xiaolong Xie¹, Yao Li¹, Chengbo Ai¹, Siyu Pu¹, Jing Chen¹

¹Department of Pediatric Surgery and Laboratory of Pediatric Surgery, West China Hospital / West China School of Medicine,Sichuan University

Journal (Developmental Biology)

Zebrafish Ovary Excision for Obtaining Granulosa Cell-Free Stage I Oocytes

Qianwen Zheng¹, Xiaolong Xie¹, Yao Li¹, Chengbo Ai¹, Siyu Pu¹, Jing Chen¹

¹Department of Pediatric Surgery and Laboratory of Pediatric Surgery, West China Hospital / West China School of Medicine,Sichuan University

Journal (Developmental Biology)

Isolation of Granulosa Cell-Free Stage I Oocytes from Zebrafish Ovaries

Qianwen Zheng¹, Xiaolong Xie¹, Yao Li¹, Chengbo Ai¹, Siyu Pu¹, Jing Chen¹

¹Department of Pediatric Surgery and Laboratory of Pediatric Surgery, West China Hospital / West China School of Medicine,Sichuan University

Journal (Developmental Biology)

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Karin Tourle¹, Amber Rucinski¹, Alexander Grainger¹, Ioannis J. Limnios¹, Macarena Gonzalez Ruiz¹, Jonathan K.H. Tan¹

¹Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine,Bond University

Journal (Developmental Biology)

Electroporated Mouse Cardiac Cell Isolation for Cell Sorting and Analysis

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

Journal (Developmental Biology)

An Efficient Transgenesis Approach for Gene Delivery in the Mouse Embryonic Heart

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

Journal (Developmental Biology)

Extraction and Electroporation of Mouse Embryo Heart as a Transgenesis Approach

Jorge Mañes-García¹, Leonardo Beccari¹, Miguel Torres^2,3, Oscar H. Ocaña^2,4

¹Tissue and Organ Homeostasis Program,Severo Ochoa Center for Molecular Biology (CBMSO), ²Cardiovascular Regeneration Program,National Cardiovascular Research Center (CNIC), ³Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), ⁴Department of Experimental Biology, Faculty of Experimental Sciences,University of Jaén

Journal (Developmental Biology)

Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies

Luigi Mancinelli^1,2, Karen Elizabeth Schoedel^3,4, Kurt Richard Weiss^5,6,7, Giuseppe Intini^1,2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, ³UPMC Presbyterian, ⁴UPMC Shadyside, ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶UPMC Hillman Cancer Center, ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, ⁸Department of Medicine,University of Pittsburgh School of Medicine, ⁹University of Pittsburgh UPMC Hillman Cancer Center, ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Methods to Enable Spatial Transcriptomics of Bone Tissues

Luigi Mancinelli^1,2, Karen Elizabeth Schoedel^3,4, Kurt Richard Weiss^5,6,7, Giuseppe Intini^1,2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, ³UPMC Presbyterian, ⁴UPMC Shadyside, ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶UPMC Hillman Cancer Center, ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, ⁸Department of Medicine,University of Pittsburgh School of Medicine, ⁹University of Pittsburgh UPMC Hillman Cancer Center, ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Generation of Germ-Free Zebrafish Models from Embryos

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

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