JOVE JOURNAL

Biology

Showing 1,045 - 1,056 in 3,287 videos.

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Journal (Biology)

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca²⁺ Transients and L-Type Calcium Current

Philipp Tomsits*^1,2,3, Dominik Schüttler*^1,2,3, Stefan Kääb^1,2, Sebastian Clauss*^1,2,3, Niels Voigt*^4,5,6

¹Department of Medicine I, University Hospital Munich, Campus Großhadern,Ludwig-Maximilians University Munich (LMU), ²Partner Site Munich, Munich Heart Alliance (MHA),DZHK (German Centre for Cardiovascular Research), ³Walter Brendel Center of Experimental Medicine,Ludwig-Maximilians University Munich (LMU), ⁴Institute of Pharmacology and Toxicology,University Medical Center Göttingen, ⁵Partner Site Göttingen,DZHK (German Centre for Cardiovascular Research), ⁶Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC),University of Göttingen

Journal (Biology)

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Kanut Laoharawee^1,2,3, Matthew J. Johnson^1,2,3, Walker S. Lahr^1,2,3, Joseph J. Peterson^1,2,3, Beau R. Webber^1,2,3, Branden S. Moriarity^1,2,3

¹Department of Pediatrics,University of Minnesota, ²Center for Genomic Engineering,University of Minnesota, ³Masonic Cancer Center,University of Minnesota

Journal (Biology)

Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

Lisa Johann^1,2, Oleksandra Chabanovska^1,2, Cajetan Immanuel Lang³, Robert David^1,2, Heiko Lemcke^1,2

¹Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC),Rostock University Medical Center, ²Faculty of Interdisciplinary Research, Department Life, Light & Matter,University Rostock, ³Department of Cardiology,Rostock University Medical Center

Journal (Biology)

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Raven Shah*¹, Shuiyun Lan*¹, Maritza N. Puray-Chavez², Dandan Liu², Philip R. Tedbury¹, Stefan G. Sarafianos¹

¹Laboratory of Biochemical Pharmacology, Department of Pediatrics,Emory University School of Medicine, ²Department of Molecular Microbiology & Immunology,University of Missouri School of Medicine

Journal (Biology)

Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

Naga S. Annamdevula^1,2, Rachel Sweat³, Hayden Gunn¹, John R. Griswold³, Andrea L. Britain^1,2, Thomas C. Rich^1,2, Silas J. Leavesley^1,2,3

¹Department of Pharmacology,University of South Alabama, ²Center for Lung Biology,University of South Alabama, ³Department of Chemical and Biomolecular Engineering,University of South Alabama

Journal (Biology)

Assessment of Global Ocular Structure Following Spaceflight Using a Micro-Computed Tomography (Micro-CT) Imaging Method

Gina D. Roque-Torres¹, Nina C. Nishiyama², Seta Stanbouly², Xiao Wen Mao²

¹Center for Dental Research,Loma Linda University, ²Department of Basic Sciences, Division of Biomedical Engineering Sciences (BMES),Loma Linda University

Journal (Biology)

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform

Shreedevi Arun Kumar¹, Jihui Lee¹, Corey J. Bishop¹

¹Department of Biomedical Engineering,Texas A&M University

Journal (Biology)

Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

Perwez Alam¹, Bryan D. Maliken¹, Malina J. Ivey¹, Shannon M. Jones¹, Onur Kanisicak¹

¹Department of Pathology and Laboratory Medicine, College of Medicine,University of Cincinnati

Journal (Biology)

Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Doğacan Yücel*^1,2, Jacob Solinsky*², Jop H. van Berlo^1,2,3

¹Department of Integrative Biology and Physiology,University of Minnesota, ²Lillehei Heart Institute, Department of Medicine,University of Minnesota, ³Stem Cell Institute,University of Minnesota

Journal (Biology)

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Dawei Xu*^1,2, Palash Chandra Mondol*¹, Muhammad Uzair¹, Matthew R. Tucker³, Dabing Zhang^1,3

¹Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, ²College of Agronomy,Anhui Agricultural University, Heifei, China, ³School of Agriculture, Food and Wine,University of Adelaide

Journal (Biology)

Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke

Katrina L. Wilson¹, S. Thomas Carmichael², Tatiana Segura^1,3,4

¹Department of Biomedical Engineering,Duke University, ²Department of Neurology,University of California Los Angeles, ³Department of Neurology,Duke University, ⁴Department of Dermatology,Duke University

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