JOVE JOURNAL

Biology

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Journal (Biology)

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

Diantha van de Vlekkert¹, Xiaohui Qiu¹, Ida Annunziata¹, Alessandra d'Azzo¹

¹Department of Genetics,St. Jude Children's Research Hospital

Journal (Biology)

Simple Detection of Primary Cilia by Immunofluorescence

Alzbeta Filipova¹, Daniel Diaz Garcia², Josef Dvorak³, Stanislav Filip⁴, Marcela Jelicova¹, Zuzana Sinkorova¹

¹Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove,University of Defence, ²Department of Clinical Biochemistry and Diagnostics,University Hospital, ³Department of Oncology, Thomayer Hospital,Charles University, ⁴Department of Oncology and Radiotherapy, Faculty of Medicine,Charles University

Journal (Biology)

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

Hedwig M. Braakhuis¹, Ruiwen He^1,2, Rob J. Vandebriel¹, Eric R. Gremmer¹, Edwin Zwart¹, Jolanda P. Vermeulen¹, Paul Fokkens¹, John Boere¹, Ilse Gosens¹, Flemming R. Cassee^1,2

¹National Institute for Public Health and the Environment (RIVM), ²Institute for Risk Assessment Sciences (IRAS)

Journal (Biology)

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Jonathan D. Partridge¹, Rasika M. Harshey¹

¹Department of Molecular Biosciences,The University of Texas at Austin

Journal (Biology)

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Jose Meseguer-Ripolles¹, Yu Wang¹, Agnes Sorteberg¹, Aishwariya Sharma², Nan-Linda Ding², Baltasar Lucendo-Villarin¹, Philipp Kramer², Charis-Patricia Segeritz², David C. Hay¹

¹MRC Centre for Regenerative Medicine,University of Edinburgh, ²Research & Development,STEMCELL Technologies Inc

Journal (Biology)

Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices

Dominik J. Fiegle¹, Tilmann Volk^1,2, Thomas Seidel^1,2

¹Institute of Cellular and Molecular Physiology,Friedrich-Alexander University Erlangen-Nürnberg, ²Muscle Research Center Erlangen (MURCE),Friedrich-Alexander-Universität Erlangen-Nürnberg

Journal (Biology)

Measuring Crop Motility and Food Passaging in Drosophila

Jiadong Cai*^1,2, Junmeng Xi*^1,2, Youheng Wei^1,2

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China,Yangzhou University, ²College of Bioscience and Biotechnology,Yangzhou University

Journal (Biology)

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Robert Beattie¹, Carmen Streicher¹, Nicole Amberg¹, Giselle Cheung¹, Ximena Contreras¹, Andi H. Hansen¹, Simon Hippenmeyer¹

¹Institute of Science and Technology Austria

Journal (Biology)

Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Łukasz Makowski¹, Damian Trojanowski¹, Jolanta Zakrzewska-Czerwińska¹

¹Department of Molecular Microbiology, Faculty of Biotechnology,University of Wroclaw

Journal (Biology)

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Jesús Espada^1,2, Elisa Carrasco³, María I. Calvo-Sánchez^1,4, Sandra Fernández-Martos¹, Juan José Montoya⁵

¹Ramón y Cajal Institute for Health Research (IRYCIS),Ramón y Cajal University Hospital, ²Centro Integrativo de Biología y Química Aplicada (CIBQA),Universidad Bernardo O'Higgins, ³Department of Molecular Biology, Faculty of Sciences,Universidad Autónoma de Madrid (UAM), ⁴Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales,Universidad Francisco de Vitoria, ⁵Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine,Complutense University

Journal (Biology)

Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer

Martijn van de Locht¹, Josine M. de Winter¹, Dilson E. Rassier², Michiel H.B. Helmes^1,3, Coen A.C. Ottenheijm¹

¹Department of Physiology,Amsterdam UMC, ²Department of Kinesiology and Physical Education, Faculty of Education,McGill University, ³IONOptix BV

Journal (Biology)

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Emily K. Matozel¹, Nathaniel Dale¹, Allen C. Price²

¹Department of Biology,Emmanuel College, ²Department of Chemistry and Physics,Emmanuel College

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