JOVE JOURNAL

Biology

Showing 997 - 1,008 in 3,287 videos.

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Raphael Nyaruaba^1,2,3, Xiaohong Li¹, Caroline Mwaliko^2,3,4, Changchang Li¹, Matilu Mwau⁵, Nelson Odiwour^1,2,3, Elishiba Muturi^1,2,3, Caroline Muema^1,2,3, Junhua Li¹, Junping Yu¹, Hongping Wei¹

¹Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology,Chinese Academy of Sciences, ²International College,University of Chinese Academy of Sciences, ³Sino-Africa Joint Research Center, ⁴CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai,Chinese Academy of Sciences, ⁵Center for Infectious and Parasitic Diseases Control Research,Kenya Medical Research Institute

Journal (Biology)

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles (“Nanoblades”)

Philippe E. Mangeot², Laura Guiguettaz¹, Thibault J. M. Sohier¹, Emiliano P. Ricci¹

¹LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, ²CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

Journal (Biology)

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants’ Extracts and Fractions to Prevent Dental Caries

Sabrina M. Ribeiro*¹, Érick D. O. Fratucelli*¹, Júlia M. Fernandes*², Paula C. P. Bueno^2,3, Alberto José Cavalheiro², Marlise I. Klein¹

¹Department of Dental Materials and Prosthodontics, School of Dentistry,São Paulo State University (UNESP), ²Department of Organic Chemistry, Institute of Chemistry,São Paulo State University (UNESP), ³Faculty of Pharmaceutical Sciences of Ribeiraão Preto (FCFRP-USP), Department of Physics and Chemistry,University of São Paulo

Journal (Biology)

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Nicole Comfort¹, Kunheng Cai², Tessa R. Bloomquist¹, Madeleine D. Strait¹, Anthony W. Ferrante Jr.², Andrea A. Baccarelli¹

¹Department of Environmental Health Sciences,Columbia University Mailman School of Public Health, ²Department of Medicine,Naomi Berrie Diabetes Center, Columbia University

Journal (Biology)

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Alejandro Lopez Gonzalez*¹, Léa Luciana*¹, Clémentine Le Nevé², Julie Valantin², Laura Francols², Nicolas Gadot², Christophe Vanbelle³, Laurianne Davignon⁴, Laura Broutier¹

¹Childhood Cancer & Cell Death (C3),Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon 69373, ²Plateforme Anatomopathologie Recherche,Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), ³Plateforme d’Imagerie cellulaire, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), ⁴PerkinElmer S.A.S.

Journal (Biology)

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

Justin A. Courson¹, Paul T. Landry¹, Thao Do¹, Eric Spehlmann², Pascal J. Lafontant², Nimesh Patel¹, Rolando E. Rumbaut^3,4, Alan R. Burns^1,4

¹College of Optometry,University of Houston, ²Department of Biology,DePauw University, ³Center for Translational Research on Inflammatory Diseases (CTRID),Michael E. DeBakey Veterans Affairs Medical Center, ⁴Children’s Nutrition Center,Baylor College of Medicine

Journal (Biology)

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

Georgios Stroulios*¹, Martin Stahl*², Fisal Elstone*², Wing Chang¹, Sharon Louis², Allen Eaves^2,3, Salvatore Simmini¹, Ryan K. Conder²

¹STEMCELL Technologies Ltd., ²STEMCELL Technologies Inc., ³Terry Fox Laboratory, BC Cancer

Journal (Biology)

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Andrea Pirosu^1,3, Lena Allweiss¹, Maura Dandri^1,2

¹Department of Internal Medicine,University Medical Center Hamburg-Eppendorf, ²German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems site, ³Research Department Virus Immunology,Leibniz Institute for Experimental Virology

Journal (Biology)

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Felix Weis¹, Wim J. H. Hagen¹, Martin Schorb², Simone Mattei^1,3

¹Structural and Computational Biology Unit,European Molecular Biology Laboratory, ²Electron Microscopy Core Facility,European Molecular Biology Laboratory, ³Imaging Centre,European Molecular Biology Laboratory

Journal (Biology)

Live-cell Imaging of Single-Cell Arrays (LISCA) – a Versatile Technique to Quantify Cellular Kinetics

Anita Reiser¹, Daniel Woschée¹, Simon Maximilian Kempe¹, Joachim Oskar Rädler¹

¹Faculty of Physics and Center for NanoScience (CeNS),Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, 80539 München, Germany

Journal (Biology)

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Johannes Groen¹, Andrea Sorrentino¹, Lucía Aballe¹, Robert Oliete¹, Ricardo Valcárcel¹, Chidinma Okolo², Ilias Kounatidis², Maria Harkiolaki², Ana Joaquina Pérez-Berná¹, Eva Pereiro¹

¹MISTRAL beamline,Alba Light Source, Cerdanyola del Valles, Spain, ²Beamline B24,Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Journal (Biology)

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Elham Vahdatahar¹, Niels Junius^1,2, Monika Budayova - Spano¹

¹CEA, CNRS, IBS,Université Grenoble Alpes, ²ELVESYS SAS

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