JOVE JOURNAL

Bioengineering

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Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Claudia Siverino¹, Barbara Tabisz², Tessa Lühmann³, Lorenz Meinel³, Thomas Müller⁴, Heike Walles^1,2, Joachim Nickel^1,2

¹Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg 'Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung',Institutsteil Würzburg, ²Lehrstuhl für Tissue Engineering und Regenerative Medizin,Universitätsklinikum Würzburg, ³Lehrstuhl für Pharmazeutische Technologie und Biopharmazie,Universität Würzburg, ⁴Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften,Universität Würzburg

Journal (Bioengineering)

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Chase A. Arbra*¹, Satish N. Nadig*¹, Sarah Grace Dennis¹, Sanket Pattanaik¹, Heather A. Bainbridge¹, J. Matthew Rhett¹, Stephen A. Fann¹, Carl Atkinson¹, Michael J. Yost¹

¹Department of Surgery,Medical University of South Carolina

Journal (Bioengineering)

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Gordon D. Hoople*¹, Andrew Richards*², Yan Wu², Albert P. Pisano³, Kun Zhang²

¹Department of Engineering,University of San Diego, ²Department of Bioengineering,University of California, San Diego, ³Department of Mechanical and Aerospace Engineering,University of California, San Diego

Journal (Bioengineering)

Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin

Kun Lv^1,2, Song Gao^1,2

¹The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School & Hospital of Stomatology,Wuhan University, ²Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology,Wuhan University

Journal (Bioengineering)

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

Simon Beaudoin¹, Michel Paquette¹, Laurent Fafard-Couture¹, Mylene A. Tremblay¹, Roger Lecomte^1,2,3, Brigitte Guérin^1,2,3, Jeffrey V. Leyton^1,2,3

¹Department of Nuclear Medicine and Radiobiology,Université de Sherbrooke, ²Sherbrooke Molecular Imaging Center (CIMS),Université de Sherbrooke, ³Sherbrooke Institute of Pharmacology

Journal (Bioengineering)

Simulating the Mechanics of Lens Accommodation via a Manual Lens Stretcher

Joshua N. Webb¹, Caroline Dong¹, Andres Bernal², Giuliano Scarcelli¹

¹Fischell Department of Bioengineering,University of Maryland, ²Bioniko Consulting LLC

Journal (Bioengineering)

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Hao-Hsiang Hsu¹, John-Kevin Kracht¹, Laura Elisabeth Harder¹, Kerstin Rudnik¹, Gerd Lindner², Katharina Schimek², Uwe Marx³, Ralf Pörtner¹

¹Institute of Bioprocess and Biosystems Engineering,Hamburg University of Technology, ²Institute of Biotechnology, Department Medical Biotechnology,Technische Universität Berlin, ³TissUse GmbH

Journal (Bioengineering)

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Madeleine S. Durkee¹, Landon D. Nash¹, Fatemeh Nooshabadi¹, Jeffrey D. Cirillo², Duncan J. Maitland¹, Kristen C. Maitland¹

¹Department of Biomedical Engineering,Texas A&M University, ²Deparment of Molecular Pathogenesis and Immunology,Texas A&M College of Medicine

Journal (Bioengineering)

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Lin Jin*^1,2, Aaron E. Engelhart*^1,3, Katarzyna P. Adamala^1,3, Jack W. Szostak¹

¹Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology,Massachusetts General Hospital, ²Department of Biomedical Engineering,Boston University, ³Department of Genetics, Cell Biology, and Development,University of Minnesota

Journal (Bioengineering)

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Melva Suarez Muñoz¹, Davide Confalonieri¹, Heike Walles^1,2, Elisabeth M. W. M. van Dongen³, Gudrun Dandekar^1,2

¹Department Tissue Engineering and Regenerative Medicine,University Hospital Wuerzburg, ²Translational Center Regenerative Therapies (TLC-RT),Fraunhofer Institute for Silicate Research ISC, ³Fujifilm Manufacturing Europe B.V.

Journal (Bioengineering)

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Tzu-Cheng Sung*¹, Hsing-Fen Li*¹, Akon Higuchi^1,2, Qing-Dong Ling^3,4, Jia-Sin Yang¹, Yeh-Chia Tseng¹, Chih-Hsien Pan Pan¹, Abdullah A. Alarfaj², Murugan A. Munusamy², Suresh Kumar⁵, Shih-Tien Hsu⁶, Kadarkarai Murugan^7,8

¹Department of Chemical and Materials Engineering,National Central University, ²Department of Botany and Microbiology,King Saud University, ³Cathay Medical Research Institute,Cathay General Hospital, ⁴Graduate Institute of Systems Biology and Bioinformatics,National Central University, ⁵Department of Medical Microbiology and Parasitology,Universiti Putra Malaysia, ⁶Department of Internal Medicine,Taiwan Landseed Hospital, ⁷Department of Zoology,Bharathiar University, ⁸Thiruvalluvar University

Journal (Bioengineering)

Mimicking and Measuring Occlusal Erosive Tooth Wear with the “Rub&Roll” and Non-contact Profilometry

Jan L. Ruben¹, Gert-Jan Truin¹, Bas A.C. Loomans¹, Marie-Charlotte D.N.J.M. Huysmans¹

¹Department of Dentistry,Radboud University Medical Center

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