JOVE JOURNAL

Bioengineering

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Sealable Femtoliter Chamber Arrays for Cell-free Biology

Sarah Elizabeth Norred^1,2, Patrick M. Caveney^1,2, Scott T. Retterer^1,2, Jonathan B. Boreyko^1,2, Jason D. Fowlkes^2,3, Charles Patrick Collier², Michael L. Simpson^1,2,3

¹Bredesen Center,University of Tennessee, Knoxville, ²Center for Nanophase Materials Sciences,Oak Ridge National Laboratory, ³Department of Materials Science and Engineering,University of Tennessee, Knoxville

Journal (Bioengineering)

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity

Xiaobao Li¹, Jisun L. Song², Alessandro Culotti¹, Wei Zhang¹, David L. Chopp³, Nanxi Lu¹, Aaron I. Packman¹

¹Department of Civil and Environmental Engineering,Northwestern University, ²Department of Chemical and Biological Engineeering,Northwestern University, ³Department of Applied Mathematics and Engineering Sciences,Northwestern University

Journal (Bioengineering)

A Microfluidic Chip for ICPMS Sample Introduction

Pascal E. Verboket¹, Olga Borovinskaya¹, Nicole Meyer¹, Detlef Günther¹, Petra S. Dittrich¹

¹Department of Chemistry and Applied Biosciences,ETH Zurich

Journal (Bioengineering)

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Angela Rossi*¹, Antje Appelt-Menzel*¹, Szymon Kurdyn¹, Heike Walles^1,2, Florian Groeber²

¹Department for Tissue Engineering and Regenerative Medicine,University Hospital Würzburg, ²Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease,Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB

Journal (Bioengineering)

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Martin D. Rees¹, Shane R. Thomas^1,2

¹Centre for Vascular Research,University of New South Wales, ²School of Medical Sciences,University of New South Wales

Journal (Bioengineering)

Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis

Stephanie M. Williams¹, Lalitha Venkataraman¹, Huilai Tian¹, Galam Khan², Brent T. Harris^2,3, Michael R. Sierks¹

¹School for Engineering of Matter, Transport and Energy,Arizona State University, ²Department of Neurology,Georgetown University Medical Center, ³Department of Pathology,Georgetown University Medical Center

Journal (Bioengineering)

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

Diana Kuehn¹, Shoaib Majeed¹, Emmanuel Guedj¹, Remi Dulize¹, Karine Baumer¹, Anita Iskandar¹, Stephanie Boue¹, Florian Martin¹, Radina Kostadinova¹, Carole Mathis¹, Nikolai V. Ivanov¹, Stefan Frentzel¹, Julia Hoeng¹, Manuel C. Peitsch¹

¹Biological Systems Research, Philip Morris International R&D,Philip Morris Products S.A.

Journal (Bioengineering)

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Thomas Reiner*¹, Jason S. Lewis¹, Brian M. Zeglis*¹

¹Department of Radiology,Memorial Sloan Kettering Cancer Center

Journal (Bioengineering)

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)

Michael Tu¹, Fang Wei¹, Jieping Yang², David Wong¹

¹School of Dentistry,University of California, Los Angeles, ²School of Medicine, Clinical Nutrition,University of California, Los Angeles

Journal (Bioengineering)

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Jianliang Shen*^1,2, Xiaoyan Wu*^1,3, Yeonju Lee¹, Joy Wolfram^1,4, Zhizhou Yang¹, Zong-Wan Mao², Mauro Ferrari^1,5, Haifa Shen^1,6

¹Department of Nanomedicine,Houston Methodist Research Institute, ²MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering,Sun Yat-sen University, ³Pediatrics Department of Union Hospital,Huazhong University of Science and Technology, ⁴CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety,National Center for Nanoscience & Technology of China, ⁵Department of Medicine,Weill Cornell Medical College, ⁶Department of Cell and Developmental Biology,Weill Cornell Medical College

Journal (Bioengineering)

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Felista L. Tansi*¹, Ronny Rüger*², Markus Rabenhold², Frank Steiniger³, Alfred Fahr², Ingrid Hilger¹

¹Experimental Radiology, Institute of Diagnostic and Interventional Radiology I,Jena University Hospital, ²Department of Pharmaceutical Technology,Friedrich-Schiller-University Jena, ³Center for Electron Microscopy,Jena University Hospital

Journal (Bioengineering)

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Lama Mulki*^1,2, J. Harry Sweigard*¹, Kip M. Connor¹

¹Angiogenesis Laboratory, Department of Ophthalmology,Harvard Medical School, Massachusetts Eye & Ear Infirmary, ²St. Elizabeth's Medical Center

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