JOVE JOURNAL

Biochemistry

Showing 97 - 108 in 778 videos.

Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster

Paula Michelotti*^1,2, Tâmie Duarte*^1,2, Cristiane L. Dalla Corte^1,2

¹Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences,Federal University of Santa Maria, ²Graduate Program in Biological Sciences: Toxicological Biochemistry,Federal University of Santa Maria

Journal (Biochemistry)

Analyzing Mitochondrial Function in a Drosophila melanogaster PINK1^B9-Null Mutant Using High-resolution Respirometry

Paula Michelotti*^1,2, Tâmie Duarte*^1,2, Cristiane L. Dalla Corte^1,2

Journal (Biochemistry)

Preparing MMA-Coated CVD Graphene for Fabrication of Graphene-Coated CryoEM Grids

Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis^1,2

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Journal (Biochemistry)

Preparing Grid-Sized MMA-Coated CVD Graphene Squares for Attaching to CryoEM Grids

Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis^1,2

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Journal (Biochemistry)

Fabricating Graphene-Coated Cryo-EM Grids Using Grid-Sized MMA-Coated CVD Graphene Squares

Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis^1,2

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Journal (Biochemistry)

Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis^1,2

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Journal (Biochemistry)

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Yi-Kai Liu¹, Zhuojun Luo¹, Anton Iliuk^1,2, Weiguo Andy Tao^1,2,3,4

¹Department of Biochemistry,Purdue University, ²Tymora Analytical Operations, ³Department of Chemistry,Purdue University, ⁴Purdue Institute for Cancer Research,Purdue University

Journal (Biochemistry)

Extracellular Vesicle (EV) Isolation from Human Urine Samples Using the EVtrap Approach

Yi-Kai Liu¹, Zhuojun Luo¹, Anton Iliuk^1,2, Weiguo Andy Tao^1,2,3,4

¹Department of Biochemistry,Purdue University, ²Tymora Analytical Operations, ³Department of Chemistry,Purdue University, ⁴Purdue Institute for Cancer Research,Purdue University

Journal (Biochemistry)

Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis

Yi-Kai Liu¹, Zhuojun Luo¹, Anton Iliuk^1,2, Weiguo Andy Tao^1,2,3,4

¹Department of Biochemistry,Purdue University, ²Tymora Analytical Operations, ³Department of Chemistry,Purdue University, ⁴Purdue Institute for Cancer Research,Purdue University

Journal (Biochemistry)

Proteome/Phosphoproteome Analysis of the Extracellular Vesicle (EV) Samples Using LC-MS/MS

Yi-Kai Liu¹, Zhuojun Luo¹, Anton Iliuk^1,2, Weiguo Andy Tao^1,2,3,4

¹Department of Biochemistry,Purdue University, ²Tymora Analytical Operations, ³Department of Chemistry,Purdue University, ⁴Purdue Institute for Cancer Research,Purdue University

Journal (Biochemistry)

A Knowledge Graph Approach to Elucidate the Role of Organellar Pathways in Disease via Biomedical Reports

Alexander R. Pelletier^1,2,3, Dylan Steinecke^1,3,4, Dibakar Sigdel¹, Irsyad Adam¹, J. Harry Caufield¹, Vladimir Guevara-Gonzalez¹, Joseph Ramirez¹, Aarushi Verma¹, Kaitlyn Bali¹, Katherine Downs¹, Wei Wang^1,2,3, Alex Bui^3,4, Peipei Ping^1,2,3,4,5

¹Department of Physiology,UCLA School of Medicine, ²Scalable Analytics Institute (ScAi) at Department of Computer Science,UCLA School of Engineering, ³NIH BRIDGE2AI Center at UCLA & NHLBI Integrated Cardiovascular Data Science Training Program,UCLA, ⁴Medical Informatics,University of California at Los Angeles (UCLA), ⁵Department of Medicine (Cardiology),UCLA School of Medicine

Journal (Biochemistry)

Extracting Venom from the Parasitoid Wasp Trichogramma dendrolimi Using an Artificial Host

Hai-yan Wang*^1,2, Ze-qi Yu*^1,2, Xin-yue Ren^1,2, Yuan-xi Li^1,2, Zhi-chao Yan^1,2,3

¹Department of Entomology,Nanjing Agricultural University, ²Key Laboratory of Integrated Pest Management of Crop of East China,Ministry of Agriculture, ³Sanya Institute of Nanjing Agricultural University

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