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Method for Culture of Early Chick Embryos ex vivo (New Culture)
JoVE Journal
Biologia
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JoVE Journal Biologia
Method for Culture of Early Chick Embryos ex vivo (New Culture)
DOI:

08:45 min

October 20, 2008

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Capítulos

  • 00:00Título
  • 00:37Introduction
  • 01:09Explanting the Embryo in Saline
  • 01:58Centering the Embryo on a Ring
  • 03:09Setting Up the Culture
  • 04:07Transferring the Culture to the Incubator
  • 05:02Fixing the Embryo
  • 07:35Representative Results/Outcome
  • 08:17Conclusion

Summary

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This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.

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