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ヒト人工多能性幹細胞からの破骨細胞の分化と特性評価
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
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ヒト人工多能性幹細胞からの破骨細胞の分化と特性評価

1,029 Views

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10:52 min

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March 22, 2024

DOI:

10.3791/66527-v

DOI:
10.3791/66527-v

•

10:52 min
March 22, 2024

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4 Views
, , , ,
1Department of Bioengineering, Department of Medicine,University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Tags

OsteoclastsHuman Induced Pluripotent Stem Cells (iPSCs)DifferentiationCharacterizationPassagingCD34+ Peripheral Blood Mononuclear CellsBone Disease ResearchPesquisa do CâncerEndoprosthesis ResearchExpandabilityDMEM/F-12DispaseHEPESCell CultureCell Aggregates

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Blümke, A., Simon, J., Leber, E., Scatena, M., Giachelli, C. M. Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (205), e66527, doi:10.3791/66527 (2024).

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