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链霉亲和素亲和网格制备用于冷冻电子显微镜样品制备

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

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链霉亲和素亲和网格制备用于冷冻电子显微镜样品制备

3,594 Views

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01:15 min

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December 29, 2023

DOI:

10.3791/66197-v

DOI:

10.3791/66197-v

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01:15 min

December 29, 2023

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32 Views

Trinity Cookis, Paul Sauer³, Simon Poepsel⁵, Bong-Gyoon Han, Dominik A. Herbst^2,6, Robert Glaeser, Eva Nogales^2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Tags

Cryo-electron Microscopy Sample Preparation Streptavidin-affinity Grid Biotinylation Two-dimensional Streptavidin Crystal Air-water Interface Sample Denaturation Preferred Orientation Sample Concentration Protein Complex Purification

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