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High-Throughput Quantification of the Synthesis and Distribution of mtDNA
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माइटोकॉन्ड्रियल डीएनए संश्लेषण और वितरण की उच्च-थ्रूपुट छवि-आधारित परिमाणीकरण
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Bioquímica
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JoVE Journal Bioquímica
High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
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माइटोकॉन्ड्रियल डीएनए संश्लेषण और वितरण की उच्च-थ्रूपुट छवि-आधारित परिमाणीकरण

1,213 Views

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01:31 min

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May 05, 2023

DOI:

10.3791/65236-v

DOI:
10.3791/65236-v

•

01:31 min
May 05, 2023

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6 Views
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1Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, 2Institute of Biochemistry and Biophysics,Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw

Tags

Mitochondrial DNADNA SynthesisDNA DistributionHigh-throughputImage-based QuantificationMitochondrial ReplicationMitochondrial MaintenanceInterferon Response PathwayOxidative Phosphorylation5-bromo-2′-deoxyuridine (BrdU) IncorporationImmunofluorescence Detection

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Borowski, L. S., Kasztelan, K., Czerwinska-Kostrzewska, J., Szczesny, R. J. High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution. J. Vis. Exp. (195), e65236, doi:10.3791/65236 (2023).

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