Journal
/
Bioquímica
/
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
/
Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

High-Throughput Quantification of the Synthesis and Distribution of mtDNA

DOI:
10.3791/65236-v

•

01:31 min

•

May 05, 2023

•

, , ,
1Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, 2Institute of Biochemistry and Biophysics,Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw

Tags

Mitochondrial DNADNA SynthesisDNA DistributionHigh-throughputImage-based QuantificationMitochondrial ReplicationMitochondrial MaintenanceInterferon Response PathwayOxidative Phosphorylation5-bromo-2′-deoxyuridine (BrdU) IncorporationImmunofluorescence Detection

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Preparation of siRNA Mixture for Mitochondrial DNA Synthesis

Cell Transfection and 5-Bromo-2'-Deoxyuridine (BrdU) Incorporation in HeLa Cells

5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells

HeLa Cell Fixation with Hoechst 33342 and Blocking

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

Read Article