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Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment (Video) | JoVE
 

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment (Video) | JoVE

Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

Article DOI: 10.3791/65206-v • 01:25 min • June 2nd, 2023
June 2nd, 2023 •
1,2,3,4, 1,2,3, 1,2,3, 1,2,3, 1,2,3,4
1Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, 2Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3Clinical Academic Center of Coimbra (CACC), 4Polytechnic Institute of Coimbra, Coimbra Health School

Tags

Fluorescent-stained Lipid Droplets 3D Reconstruction Hepatic Steatosis Assessment Histological Staining Microvesicular Steatosis Oil Red Pro-BODIPY Hydrophobicity Lipid Droplet Tagging Environment Polarity Immunochemistry Techniques Subcellular Antigens Lipid Droplet Biogenesis Lipid Droplet Dynamics Microvesicular Hepatic Steatosis
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