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The Serial Anesthesia Array for the High-Throughput Investigation of Volatile Agents Using Drosophila melanogaster
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Genética
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JoVE Journal Genética
The Serial Anesthesia Array for the High-Throughput Investigation of Volatile Agents Using Drosophila melanogaster

The Serial Anesthesia Array for the High-Throughput Investigation of Volatile Agents Using Drosophila melanogaster

DOI:
10.3791/65144-v

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06:27 min

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February 24, 2023

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, , ,
1Department of Anesthesiology,University of Wisconsin-Madison, 2Department of Medical Genetics,University of Wisconsin-Madison

Capítulos

  • 00:04Introduction
  • 00:52Construction of the SAA
  • 03:40Operation of the SAA
  • 04:34Checklist Prior to Starting an Experiment
  • 05:06Results: Effect of Genotype on Behavioral Sensitivity to Anesthetics
  • 05:49Conclusion

Summary

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The fruit fly (Drosophila melanogaster) is widely used for biological and toxicological research. To expand the utility of flies, we developed an instrument, the serial anesthesia array, that simultaneously exposes multiple fly samples to volatile general anesthetics (VGAs), making it possible to investigate the collateral effects (toxic and protective) of VGAs.

Tags

Serial Anesthesia ArrayVolatile AgentsDrosophila MelanogasterPharmacogeneticsAnesthesiaHigh-throughput ProcessingGenetic ToolboxVolatile AgentsPollutantsTherapeutic AgentsToxic EffectsBeneficial EffectsGenéticaWooden Frame50 Ml Conical Tube Caps5 Ml Serological PipettesNettingAdhesive Cable Tie DownsTygon TubingFlow IndicatorVaporizerCarrier Gas TankRegulator

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