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使用 体内 显微注射和电穿孔的体细胞基因组工程小鼠模型
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation
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使用 体内 显微注射和电穿孔的体细胞基因组工程小鼠模型

DOI:
10.3791/65131-v

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08:06 min

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May 05, 2023

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, ,
1Rosalind and Morris Goodman Cancer Research Institute,McGill University, 2Department of Human Genetics,McGill University, 3McGill Integrated Core for Animal Modeling (MICAM),McGill University

Capítulos

  • 00:05Introduction
  • 01:01Preparing Microinjection Needle, Surgery Area, Solution, and Needle for Injection
  • 02:26Exposing the Female Reproductive Tract
  • 04:11In Vivo Oviduct Injection and Electroporation
  • 06:29Results: Validation of Successful In Vivo Injection and Electroporation of Oviduct Epithelial Cells
  • 07:16Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了显微注射和 体内 电穿孔，用于小鼠输卵管中区域限制的CRISPR介导的基因组编辑。

Tags

Somatic Genome EngineeringMouse ModelsIn Vivo MicroinjectionElectroporationOrgan TargetingCancer InitiationGenome ManipulationImmunocompetent EnvironmentAdaptable Mutation CombinationsOviduct TargetingOrgan Parenchyma TargetingGlass Capillary MicroinjectionAnesthesiaAseptic Technique

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